Supercritical fluid chromatography-mass spectrometry enables simultaneous measurement of all phosphoinositide regioisomers

Abstract

Phosphoinositide (PIP) species play important roles in a number of cellular events, but the simultaneous quantification of individual PIP species remains challenging using conventional methods. Here, the authors report a supercritical fluid chromatography-mass spectrometry approach to simultaneous measure the molecular species of all seven PIP regioisomers.