lncRNA KLF3-AS1 Suppresses Cell Migration and Invasion in ESCC by Impairing miR-185-5p-Targeted KLF3 Inhibition

Jun-Qi Liu; Ming Deng; Nan-Nan Xue; Ting-Xuan Li; Yue-Xin Guo; Liang Gao; Di Zhao; Rui-Tai Fan

Molecular Therapy: Nucleic Acids (Jun 2020)

lncRNA KLF3-AS1 Suppresses Cell Migration and Invasion in ESCC by Impairing miR-185-5p-Targeted KLF3 Inhibition

Jun-Qi Liu,
Ming Deng,
Nan-Nan Xue,
Ting-Xuan Li,
Yue-Xin Guo,
Liang Gao,
Di Zhao,
Rui-Tai Fan

Affiliations

Jun-Qi Liu: Department of Radiotherapy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, P.R. China
Ming Deng: Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430060, P.R. China
Nan-Nan Xue: Department of Radiotherapy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, P.R. China
Ting-Xuan Li: Department of Radiotherapy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, P.R. China
Yue-Xin Guo: Department of Radiotherapy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, P.R. China
Liang Gao: Center of Experimental Orthopaedics, Saarland University, Kirrberger Strasse, Building 37, 66421 Homburg, Germany
Di Zhao: Department of Endocrinology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, P.R. China; Corresponding author: Di Zhao, Department of Endocrinology, The First Affiliated Hospital of Zhengzhou University, No. 1, Jianshe East Road, Erqi District, Zhengzhou 450000, Henan Province, P.R. China.
Rui-Tai Fan: Department of Radiotherapy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, P.R. China; Corresponding author: Rui-Tai Fan, Department of Radiotherapy, The First Affiliated Hospital of Zhengzhou University, No. 1, Jianshe East Road, Erqi District, Zhengzhou 450000, Henan Province, P.R. China.

Journal volume & issue: Vol. 20
pp. 231 – 241

Abstract

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Esophageal squamous cell carcinoma (ESCC) is a common cancer occurring in males and females worldwide. Accumulating evidence continues to highlight the crucial roles of long non-coding RNAs (lncRNAs) in the process of tumorigenesis. However, the regulatory mechanism of lncRNAs in ESCC remains unclear. The aim of this study is to elucidate the role of lncRNA Krüppel-like factor 3 antisense RNA 1 (KLF3-AS1) in ESCC by regulating miR-185-5p and KLF3. Initially, ESCC cell spheres with stem cell-like properties were prepared by suspension culture, and subsequently characterized by assessing colony formation ability and stem cell markers. LncRNA KLF3-AS1 was found to be poorly expressed in ESCC and could upregulate the expression of KLF3 by binding to miR-185-5p. lncRNA KLF3-AS1 upregulation was observed to inhibit miR-185-5p, thereby contributing to decreased expression of SOX2 and Oct4 (octamer-binding transcription factor 4). Furthermore, enhancement of lncRNA KLF3-AS1 resulted in reduced colony formation ability, cell invasion and migration, and tumor volume in vivo while promoting cell apoptosis in ESCC through downregulation of miR-185-5p. Collectively, this study indicated that lncRNA KLF3-AS1 inhibited ESCC cell invasion and migration by impairing miR-185-5p-mediated inhibition of KLF3, highlighting a promising novel potential target for ESCC treatment.

Published in Molecular Therapy: Nucleic Acids

ISSN: 2162-2531 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: https://www.cell.com/molecular-therapy-family/nucleic-acids/latest-content

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