OXIDATIVE STRESS AND STATE OF STRESS-RESPONDING: PATHOGENETIC PARTNERSHIP FOR COMMUNITY-ACQUIRED PNEUMONIA IN CHILDREN

Abstract

Despite the significant progress made in studying the pathogenesis, diagnosis and treatment of community-acquired pneumonia in children, until now there has been no significant change in the morbidity and mortality rates of this pathology. This leads to the search for new markers of the pneumonia course severity, the detection of comorbid conditions, and the study of their effect on the child's body. The aim of the study ‒ to learn the relationship between changes in the state of stress-sensitive systems and antioxidant protection in oxidative stress in children with community-acquired pneumonia. Materials and Methods. Seventy children with community-acquired pneumonia were examined. The state of the antioxidant defense system, the state of stress-dependent systems, apoptosis and the level of molecular damage were assessed. Results and Discussion. When examining patients with pneumonia of moderate severity and severe course, significantly significant changes in glutathione and free radical oxidation systems were detected, characterized by a decrease in GSH content of 6.01 % and 12.27 %, respectively, and an increase in GSH content by 24.12 % and 65.52 % respectively. In severe pneumonia, SOD activity decreased at the same time (2.04 (1.61, 3.51) CU/mg protein)) and reduced redox-glutathione index to 0.817 (0.805–0.829) CU, against the background of an increase in the content of oxyradicals, caspase-3, the intensive formation of oxidative modification of proteins and an increased DNA fragmentation. The revealed changes tend to decrease depending on the severity of pneumonia. Conclusions. Fluctuations in the indices of oxidative stress, the level of molecular damage and the intensity of apoptosis in children with out-of-hospital pneumonia correlate with the severity of the disease.

Keywords

• позалікарняна пневмонія
• окисний стрес
• цитотоксичність
• апоптоз.