Microorganisms (Dec 2022)
Identification and Molecular Characterization of Four New <i>Blastocystis</i> Subtypes Designated ST35-ST38
- Jenny G. Maloney,
- Aleksey Molokin,
- Raimundo Seguí,
- Pablo Maravilla,
- Fernando Martínez-Hernández,
- Guiehdani Villalobos,
- Anastasios D. Tsaousis,
- Eleni Gentekaki,
- Carla Muñoz-Antolí,
- Debora R. Klisiowicz,
- Camila Y. Oishi,
- Rafael Toledo,
- J. Guillermo Esteban,
- Pamela C. Köster,
- Aida de Lucio,
- Alejandro Dashti,
- Begoña Bailo,
- Rafael Calero-Bernal,
- David González-Barrio,
- David Carmena,
- Mónica Santín
Affiliations
- Jenny G. Maloney
- Environmental Microbial and Food Safety Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA
- Aleksey Molokin
- Environmental Microbial and Food Safety Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA
- Raimundo Seguí
- Department of Pharmacy, Pharmaceutical Technology and Parasitology, Parasitology Area, Faculty of Pharmacy, Valencia University, Avda. Vicent Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain
- Pablo Maravilla
- Departamento de Ecologia de Agentes Patogenos, Hospital General Dr. Manuel Gea Gonzalez, Mexico City 14080, Mexico
- Fernando Martínez-Hernández
- Departamento de Ecologia de Agentes Patogenos, Hospital General Dr. Manuel Gea Gonzalez, Mexico City 14080, Mexico
- Guiehdani Villalobos
- Departamento de Ecologia de Agentes Patogenos, Hospital General Dr. Manuel Gea Gonzalez, Mexico City 14080, Mexico
- Anastasios D. Tsaousis
- Laboratory of Molecular and Evolutionary Parasitology, RAPID Group, School of Biosciences, University of Kent, Canterbury CT2 7NJ, UK
- Eleni Gentekaki
- School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand
- Carla Muñoz-Antolí
- Department of Pharmacy, Pharmaceutical Technology and Parasitology, Parasitology Area, Faculty of Pharmacy, Valencia University, Avda. Vicent Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain
- Debora R. Klisiowicz
- Department of Basic Pathology, Biological Sciences Area, Paraná Federal University, Av. Cel. Francisco H. dos Santos 100, Curitiba 19031, Brazil
- Camila Y. Oishi
- Department of Basic Pathology, Biological Sciences Area, Paraná Federal University, Av. Cel. Francisco H. dos Santos 100, Curitiba 19031, Brazil
- Rafael Toledo
- Department of Pharmacy, Pharmaceutical Technology and Parasitology, Parasitology Area, Faculty of Pharmacy, Valencia University, Avda. Vicent Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain
- J. Guillermo Esteban
- Department of Pharmacy, Pharmaceutical Technology and Parasitology, Parasitology Area, Faculty of Pharmacy, Valencia University, Avda. Vicent Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain
- Pamela C. Köster
- Parasitology Reference and Research Laboratory, Spanish National Centre for Microbiology, Majadahonda, 28222 Madrid, Spain
- Aida de Lucio
- Parasitology Reference and Research Laboratory, Spanish National Centre for Microbiology, Majadahonda, 28222 Madrid, Spain
- Alejandro Dashti
- Parasitology Reference and Research Laboratory, Spanish National Centre for Microbiology, Majadahonda, 28222 Madrid, Spain
- Begoña Bailo
- Parasitology Reference and Research Laboratory, Spanish National Centre for Microbiology, Majadahonda, 28222 Madrid, Spain
- Rafael Calero-Bernal
- SALUVET, Department of Animal Health, Faculty of Veterinary, Complutense University of Madrid, 28040 Madrid, Spain
- David González-Barrio
- Parasitology Reference and Research Laboratory, Spanish National Centre for Microbiology, Majadahonda, 28222 Madrid, Spain
- David Carmena
- Parasitology Reference and Research Laboratory, Spanish National Centre for Microbiology, Majadahonda, 28222 Madrid, Spain
- Mónica Santín
- Environmental Microbial and Food Safety Laboratory, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA
- DOI
- https://doi.org/10.3390/microorganisms11010046
- Journal volume & issue
-
Vol. 11,
no. 1
p. 46
Abstract
Three recent studies of Blastocystis epidemiology in mammalian hosts identified four novel sequences that appeared to share B. lapemi as the most similar sequence. However, full-length ssu rRNA gene sequences were not available to confirm the validity of these new subtypes. In the present study, Nanopore MinION sequencing was used to obtain full-length reference sequences for each of the new subtypes. Additionally, phylogenetic analyses and pairwise distance comparisons were performed to confirm the validity of each of these new subtypes. We propose that the novel sequences described in this study should be assigned the subtype designations ST35-ST38. The full-length reference sequences of ST35-ST38 will assist in accurate sequence descriptions in future studies of Blastocystis epidemiology and subtype diversity.
Keywords
