Impact of coexisting type 2 diabetes mellitus on the urinary microbiota of kidney stone patients
Xiang Li,
Yifan Tang,
Zhenyi Xu,
Hao Lin,
Shichao Wei,
Jiayi Sheng,
Lei Hu,
Shiyu Wang,
Yu Zhao,
Zhi Li,
Chaowei Fu,
Yifeng Gu,
Qun Wei,
Fengping Liu,
Ninghan Feng,
Weiguo Chen
Affiliations
Xiang Li
Department of Urology, The First Affiliated Hospital of Soochow University, Suzhou, China
Yifan Tang
Department of Urology, Affiliated Wuxi No. 2 Hospital, Nanjing Medical University, Wuxi, China
Zhenyi Xu
Department of Urology, Affiliated Wuxi No. 2 Hospital, Nanjing Medical University, Wuxi, China
Hao Lin
Department of Urology, Affiliated Wuxi No. 2 Hospital, Nanjing Medical University, Wuxi, China
Shichao Wei
Department of Urology, Affiliated Wuxi No. 2 Hospital, Nanjing Medical University, Wuxi, China
Jiayi Sheng
Department of Urology, Affiliated Wuxi No. 2 Hospital, Nanjing Medical University, Wuxi, China
Lei Hu
Department of Urology, Affiliated Wuxi No. 2 Hospital, Nanjing Medical University, Wuxi, China
Shiyu Wang
Wuxi School of Medicine, Jiangnan University, Wuxi, China
Yu Zhao
Wuxi School of Medicine, Jiangnan University, Wuxi, China
Zhi Li
Wuxi School of Medicine, Jiangnan University, Wuxi, China
Chaowei Fu
Wuxi School of Medicine, Jiangnan University, Wuxi, China
Yifeng Gu
Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, School of Medicine, The First Affiliated Hospital, Zhejiang University, Hangzhou, China
Qun Wei
Department of Surgical Oncology, Institute of Clinical Medicine, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou, China
Fengping Liu
Wuxi School of Medicine, Jiangnan University, Wuxi, China
Ninghan Feng
Department of Urology, Affiliated Wuxi No. 2 Hospital, Nanjing Medical University, Wuxi, China
Weiguo Chen
Department of Urology, The First Affiliated Hospital of Soochow University, Suzhou, China
Objectives Type 2 diabetes mellitus (T2DM) commonly complicates kidney stone disease (KSD). Our objective is to investigate the variations in the urinary microbiota between individuals with KSD alone and those with KSD plus T2DM. This exploration could have implications for disease diagnosis and treatment strategies. Methods During lithotripsy, a ureterscope was employed, and 1 mL of urine was collected from the renal pelvis after bladder disinfection. Sequencing targeting the V3–V4 hypervariable region was performed using the 16S rRNA and Illumina Novaseq platform. Results The Shannon index showed a significant decrease in the KSD plus T2DM group compared to the KSD-only group (false discovery rate = 0.041). Principal Coordinate Analysis (PCoA) demonstrated a distinct bacterial community in the KSD plus T2DM group compared to the KSD-only group (false discovery rate = 0.027). The abundance of Sphingomonas, Corynebacterium, and Lactobacillus was significantly higher in the KSD plus T2DM group than in the KSD-only group (false discovery rate < 0.05). Furthermore, Enhydrobacter, Chryseobacterium, and Allobaculum were positively correlated with fasting blood glucose and HbA1c values (P < 0.05). Conclusions The urinary microbiota in the renal pelvis exhibits differences between patients with KSD plus T2DM and those with KSD alone. Further studies employing animal models are necessary to validate these distinctions, potentially paving the way for therapeutic developments based on the urinary microbiota.
