Nature Communications (Jul 2022)
Homogeneous surrogate virus neutralization assay to rapidly assess neutralization activity of anti-SARS-CoV-2 antibodies
- Sun Jin Kim,
- Zhong Yao,
- Morgan C. Marsh,
- Debra M. Eckert,
- Michael S. Kay,
- Anna Lyakisheva,
- Maria Pasic,
- Aiyush Bansal,
- Chaim Birnboim,
- Prabhat Jha,
- Yannick Galipeau,
- Marc-André Langlois,
- Julio C. Delgado,
- Marc G. Elgort,
- Robert A. Campbell,
- Elizabeth A. Middleton,
- Igor Stagljar,
- Shawn C. Owen
Affiliations
- Sun Jin Kim
- Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah
- Zhong Yao
- Donnelly Centre, University of Toronto
- Morgan C. Marsh
- Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah
- Debra M. Eckert
- Department of Biochemistry, University of Utah School of Medicine
- Michael S. Kay
- Department of Biochemistry, University of Utah School of Medicine
- Anna Lyakisheva
- Donnelly Centre, University of Toronto
- Maria Pasic
- Department of Laboratory Medicine & Pathobiology, University of Toronto
- Aiyush Bansal
- Centre for Global Health Research, Unity Health Toronto, University of Toronto
- Chaim Birnboim
- Centre for Global Health Research, Unity Health Toronto, University of Toronto
- Prabhat Jha
- Centre for Global Health Research, Unity Health Toronto, University of Toronto
- Yannick Galipeau
- Department of Biochemistry, Microbiology & Immunology, Faculty of Medicine, University of Ottawa
- Marc-André Langlois
- Department of Biochemistry, Microbiology & Immunology, Faculty of Medicine, University of Ottawa
- Julio C. Delgado
- ARUP Institute for Clinical and Experimental Pathology
- Marc G. Elgort
- ARUP Institute for Clinical and Experimental Pathology
- Robert A. Campbell
- Department of Pathology, University of Utah
- Elizabeth A. Middleton
- Department of Internal Medicine, Division of General Medicine, University of Utah
- Igor Stagljar
- Donnelly Centre, University of Toronto
- Shawn C. Owen
- Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah
- DOI
- https://doi.org/10.1038/s41467-022-31300-9
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 9
Abstract
Neutralisation assays are key to understanding immune responses to SARS-CoV-2 infection or vaccination. Here, the authors report a surrogate virus neutralization assay called Neu-SATiN, which measures neutralization directly from sera, and allows easy adaptation to variant-specific testing.
