Investigating the mechanism of action of hemofugal and blood-stasis-expelling soup in the treatment of coronary heart disease based on network pharmacology

Abstract

Objective: To investigate the mechanism of action of Xuefu yuyuxu Tang in the treatment of Coronary Heart Disease (CHD) based on network-based pharmacology. Methods: Traditional Chinese Medicine Systems Pharmacology Databaseand Analysis Platform (TCMSP) was used to retrieve the active ingredients and targets of Chinese medicines in Xufu yuyuxu Tang. Cytoscape3.7.2 was used to draw the network diagram of “TCM-active ingredients-target genes”, and Slring database was used to construct the protein-protein interaction (PPI) network diagram, and the PPI network diagram was analyzed with the help of CyoNCA plug-in for network topology analysis, and the key targets were screened with the help of Centiseape plug-in, and the key targets were selected with the help of Centiseape plug-in. The PPI network map was analyzed by CyoNCA plug-in for network topology analysis, and Centiseape plug-in was used to screen the key targets, and MetaScape database was used for GO function analysis and KEGG pathway enrichment analysis. Molecular docking prediction was performed by AutoDockTools-1.5.6. Results: By searching TCMSP, 195 active ingredients were screened, and the pathways related to coronary heart disease were lipid and atherosclerosis, hypoxia-inducible factor-1, relaxin, and nuclear factor-KB signaling pathway, etc. The results of topology analysis showed that the target genes with the top 10 residences of Desree value were, in order, PTGS2MMP2, VEGFA, AKTI, ALB, CASP3, STAT3, II6, MM9SERPINEI.The main active ingredients of this formula, lignocaine, kaempferol, naringenin, bound well to the target proteins protein kinase 1, albumin and insulin. Conclusion: Based on the network pharmacology, this paper analyzed the active ingredients and their mechanism of action in the treatment of coronary artery disease with blood-fu chasing blood stasis soup, and found that blood-fu chasing blood stasis soup treats coronary artery disease through multi-targets, and several ingredients showed good activity, which provided a theoretical basis for the further study of the mechanism of action of the formula.