Backbone-independent NMR resonance assignments of methyl probes in large proteins

Santrupti Nerli; Viviane S. De Paula; Andrew C. McShan; Nikolaos G. Sgourakis

doi:10.1038/s41467-021-20984-0

Nature Communications (Jan 2021)

Backbone-independent NMR resonance assignments of methyl probes in large proteins

Santrupti Nerli,
Viviane S. De Paula,
Andrew C. McShan,
Nikolaos G. Sgourakis

Affiliations

Santrupti Nerli: Department of Biomolecular Engineering, University of California
Viviane S. De Paula: Center for Computational and Genomic Medicine, Department of Pathology and Laboratory Medicine, The Children’s Hospital of Philadelphia and Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania
Andrew C. McShan: Center for Computational and Genomic Medicine, Department of Pathology and Laboratory Medicine, The Children’s Hospital of Philadelphia and Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania
Nikolaos G. Sgourakis: Center for Computational and Genomic Medicine, Department of Pathology and Laboratory Medicine, The Children’s Hospital of Philadelphia and Department of Biochemistry and Biophysics, Perelman School of Medicine, University of Pennsylvania

DOI: https://doi.org/10.1038/s41467-021-20984-0
Journal volume & issue: Vol. 12, no. 1
pp. 1 – 13

Abstract

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Here, the authors present Methyl Assignments Using Satisfiability (MAUS), a method for the assignment of methyl groups using raw NOE data. They use eight proteins in the 10–45 kDa size range as test cases and show that MAUS yields 100% accurate assignments at high completeness levels.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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