Single molecule analysis reveals monomeric XPA bends DNA and undergoes episodic linear diffusion during damage search

Emily C. Beckwitt; Sunbok Jang; Isadora Carnaval Detweiler; Jochen Kuper; Florian Sauer; Nina Simon; Johanna Bretzler; Simon C. Watkins; Thomas Carell; Caroline Kisker; Bennett Van Houten

doi:10.1038/s41467-020-15168-1

Nature Communications (Mar 2020)

Single molecule analysis reveals monomeric XPA bends DNA and undergoes episodic linear diffusion during damage search

Emily C. Beckwitt,
Sunbok Jang,
Isadora Carnaval Detweiler,
Jochen Kuper,
Florian Sauer,
Nina Simon,
Johanna Bretzler,
Simon C. Watkins,
Thomas Carell,
Caroline Kisker,
Bennett Van Houten

Affiliations

Emily C. Beckwitt: Program in Molecular Biophysics and Structural Biology, University of Pittsburgh
Sunbok Jang: UPMC Hillman Cancer Center
Isadora Carnaval Detweiler: Department of Chemical Engineering, University of Pittsburgh
Jochen Kuper: Rudolf Virchow Center for Experimental Biomedicine, Institute for Structural Biology, University of Würzburg
Florian Sauer: Rudolf Virchow Center for Experimental Biomedicine, Institute for Structural Biology, University of Würzburg
Nina Simon: Center for Integrated Protein Science at the Department of Chemistry, Ludwig Maximillian University of Munich
Johanna Bretzler: Center for Integrated Protein Science at the Department of Chemistry, Ludwig Maximillian University of Munich
Simon C. Watkins: Center for Biologic Imaging, University of Pittsburgh
Thomas Carell: Center for Integrated Protein Science at the Department of Chemistry, Ludwig Maximillian University of Munich
Caroline Kisker: Rudolf Virchow Center for Experimental Biomedicine, Institute for Structural Biology, University of Würzburg
Bennett Van Houten: UPMC Hillman Cancer Center

DOI: https://doi.org/10.1038/s41467-020-15168-1
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 14

Abstract

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It is not fully understood how XPA interacts with a DNA lesion during nucleotide excision repair. Here, the authors use single molecule analysis to study XPA–DNA interactions, including the DNA bend angle, protein stoichiometry, and diffusive properties during damage search.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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