Hydroxyapatite/L-Lysine Composite Coating as Glassy Carbon Electrode Modifier for the Analysis and Detection of Nile Blue A

Jimmy Julio Kouanang Ngouoko; Kevin Yemele Tajeu; Ranil Clément Tonleu Temgoua; Giscard Doungmo; Ingo Doench; Arnaud Kamdem Tamo; Théophile Kamgaing; Anayancy Osorio-Madrazo; Ignas Kenfack Tonle

doi:10.3390/ma15124262

Materials (Jun 2022)

Hydroxyapatite/L-Lysine Composite Coating as Glassy Carbon Electrode Modifier for the Analysis and Detection of Nile Blue A

Jimmy Julio Kouanang Ngouoko,
Kevin Yemele Tajeu,
Ranil Clément Tonleu Temgoua,
Giscard Doungmo,
Ingo Doench,
Arnaud Kamdem Tamo,
Théophile Kamgaing,
Anayancy Osorio-Madrazo,
Ignas Kenfack Tonle

Affiliations

Jimmy Julio Kouanang Ngouoko: Department of Chemistry, Faculty of Science, University of Dschang, Dschang P.O. Box 67, Cameroon
Kevin Yemele Tajeu: Department of Chemistry, Faculty of Science, University of Dschang, Dschang P.O. Box 67, Cameroon
Ranil Clément Tonleu Temgoua: Department of Chemistry, Faculty of Science, University of Dschang, Dschang P.O. Box 67, Cameroon
Giscard Doungmo: Institute of Inorganic Chemistry, Christian-Albrechts-Universität zu Kiel, Max-Eyth-Straβe 2, 24118 Kiel, Germany
Ingo Doench: Laboratory for Bioinspired Materials BMBT, Institute of Microsystems Engineering IMTEK-Sensors, University of Freiburg, 79110 Freiburg, Germany
Arnaud Kamdem Tamo: Laboratory for Bioinspired Materials BMBT, Institute of Microsystems Engineering IMTEK-Sensors, University of Freiburg, 79110 Freiburg, Germany
Théophile Kamgaing: Department of Chemistry, Faculty of Science, University of Dschang, Dschang P.O. Box 67, Cameroon
Anayancy Osorio-Madrazo: Laboratory for Bioinspired Materials BMBT, Institute of Microsystems Engineering IMTEK-Sensors, University of Freiburg, 79110 Freiburg, Germany
Ignas Kenfack Tonle: Department of Chemistry, Faculty of Science, University of Dschang, Dschang P.O. Box 67, Cameroon

DOI: https://doi.org/10.3390/ma15124262
Journal volume & issue: Vol. 15, no. 12
p. 4262

Abstract

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An amperometric sensor was developed by depositing a film coating of hydroxyapatite (HA)/L-lysine (Lys) composite material on a glassy carbon electrode (GCE). It was applied for the detection of Nile blue A (NBA). Hydroxyapatite was obtained from snail shells and its structural properties before and after its combination with Lys were characterized using X-ray diffraction (XRD), Fourier-transform infrared (FTIR) spectroscopy, scanning electron microscopy (SEM), and Brunauer–Emmett–Teller (BET) surface area analyses. The coupling of Lys to HA was attributed to favorable interaction between negatively charged -COO− groups of Lys and divalent ions Ca2+ of HA. Electrochemical investigations pointed out the improvement in sensitivity of the GCE/Lys/HA sensor towards the detection of NBA in solution. The dependence of the peak current and potential on the pH, scan rate, and NBA concentration was also investigated. Under optimal conditions, the GCE/Lys/HA sensor showed a good reproducibility, selectivity, and a NBA low detection limit of 5.07 × 10−8 mol L−1. The developed HA/Lys-modified electrode was successfully applied for the detection of NBA in various water samples.

Published in Materials

ISSN: 1996-1944 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Technology: Electrical engineering. Electronics. Nuclear engineering; Technology: Engineering (General). Civil engineering (General); Science: Natural history (General): Microscopy; Science: Physics: Descriptive and experimental mechanics
Website: http://www.mdpi.com/journal/materials/

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