Binging from Food to Alcohol: A Sequential Interaction Between Binging Behaviors in Male Wistar Rats
Sergio Cuesta-Martínez,
Leandro Ruiz-Leyva,
Ana Jiménez-García,
Teresa Aparicio-Mescua,
Olga López-Guarnido,
Ricardo Pautassi,
Ignacio Morón,
Cruz Cendán
Affiliations
Sergio Cuesta-Martínez
Department of Pharmacology, Biomedical Research Center (CIBM) and Institute of Neuroscience, Faculty of Medicine, University of Granada, Parque Tecnológico de Ciencias de la Salud, 18016, Granada, SpainBiosanitary Research Institute, University Hospital Complex of Granada, 18012, Granada, Spain
Leandro Ruiz-Leyva
Department of Pharmacology, Biomedical Research Center (CIBM) and Institute of Neuroscience, Faculty of Medicine, University of Granada, Parque Tecnológico de Ciencias de la Salud, 18016, Granada, SpainBiosanitary Research Institute, University Hospital Complex of Granada, 18012, Granada, Spain
Ana Jiménez-García
Department of Psychology, Faculty of Life Sciences and Nature, University of Nebrija, Hoyo de Manzanares, 28240, Madrid, Spain
Teresa Aparicio-Mescua
Department of Psychobiology and Centre of Investigation of Mind, Brain, and Behavior (CIMCYC), Faculty of Psychology, University of Granada, Campus Cartuja, 18071, Granada, Spain
Olga López-Guarnido
Department of Legal Medicine and Toxicology, Faculty of Medicine, University of Granada, Parque Tecnológico de Ciencias de la Salud, 18016, Granada, Spain
Ricardo Pautassi
Instituto de Investigación Médica M. y M. Ferreyra (INIMEC – CONICET-Universidad Nacional de Córdoba), Córdoba, 5000, Argentina, Faculty of Psychology, UNC, Córdoba, Argentina
Ignacio Morón
Department of Psychobiology and Centre of Investigation of Mind, Brain, and Behavior (CIMCYC), Faculty of Psychology, University of Granada, Campus Cartuja, 18071, Granada, Spain
Cruz Cendán
Department of Pharmacology, Biomedical Research Center (CIBM) and Institute of Neuroscience, Faculty of Medicine, University of Granada, Parque Tecnológico de Ciencias de la Salud, 18016, Granada, SpainBiosanitary Research Institute, University Hospital Complex of Granada, 18012, Granada, Spain
The development of excessive alcohol (ethanol) and/or highly palatable food self-administration is an essential task to elucidate the neurobiological mechanisms that underlie these behaviors. Previous work has highlighted that ethanol self-administration is modulated by both the induction of aversive states (i.e., stress or frustration) and by the concurrent availability of appetitive stimuli (e.g., food). In our protocol, rats are food deprived for three days until they reach 82%–85% of their ad libitum weight. After that, rats are exposed daily for 10 days to a brief binge or control eating experience with highly sugary and palatable food (i.e., the ingestion of 11.66 and 0.97 kcal/3 min, respectively), which is followed by a two-bottle-choice test (ethanol vs. water) in their home cages for 90 min. This model induces robust binge eating, which is followed by a selective increase in ethanol self-administration. Therefore, this protocol allows to study: a) behavioral and neurobiological factors related to binge eating, b) different stages of alcohol use, and c) interactions between the latter and other addictive-like behaviors, like binge eating.
