Integrated Analysis of Transcriptome and Metabolome Reveals the Mechanism of Chlorine Dioxide Repressed Potato (Solanum tuberosum L.) Tuber Sprouting

Xiaoyuan Zheng; Mei Li; Mei Li; Shilong Tian; Shilong Tian; Shouqiang Li; Shouqiang Li; Jianxin Chen; Jianxin Chen; Xuejiao Zhang; Xiaohua Wu; Xiaohua Wu; Xia Ge; Xia Ge; Jiachun Tian; Jiachun Tian; Yuwen Mu; Yuwen Mu; Juan Song; Juan Song

doi:10.3389/fpls.2022.887179

Frontiers in Plant Science (May 2022)

Integrated Analysis of Transcriptome and Metabolome Reveals the Mechanism of Chlorine Dioxide Repressed Potato (Solanum tuberosum L.) Tuber Sprouting

Xiaoyuan Zheng,
Mei Li,
Mei Li,
Shilong Tian,
Shilong Tian,
Shouqiang Li,
Shouqiang Li,
Jianxin Chen,
Jianxin Chen,
Xuejiao Zhang,
Xiaohua Wu,
Xiaohua Wu,
Xia Ge,
Xia Ge,
Jiachun Tian,
Jiachun Tian,
Yuwen Mu,
Yuwen Mu,
Juan Song,
Juan Song

Affiliations

Xiaoyuan Zheng: College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China
Mei Li: Agricultural Product Storage and Processing Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, China
Mei Li: Gansu Innovation Center of Fruit and Vegetable Storage and Processing, Lanzhou, China
Shilong Tian: Agricultural Product Storage and Processing Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, China
Shilong Tian: Gansu Innovation Center of Fruit and Vegetable Storage and Processing, Lanzhou, China
Shouqiang Li: Agricultural Product Storage and Processing Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, China
Shouqiang Li: Gansu Innovation Center of Fruit and Vegetable Storage and Processing, Lanzhou, China
Jianxin Chen: Agricultural Product Storage and Processing Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, China
Jianxin Chen: Gansu Innovation Center of Fruit and Vegetable Storage and Processing, Lanzhou, China
Xuejiao Zhang: College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China
Xiaohua Wu: Agricultural Product Storage and Processing Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, China
Xiaohua Wu: Gansu Innovation Center of Fruit and Vegetable Storage and Processing, Lanzhou, China
Xia Ge: Agricultural Product Storage and Processing Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, China
Xia Ge: Gansu Innovation Center of Fruit and Vegetable Storage and Processing, Lanzhou, China
Jiachun Tian: Agricultural Product Storage and Processing Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, China
Jiachun Tian: Gansu Innovation Center of Fruit and Vegetable Storage and Processing, Lanzhou, China
Yuwen Mu: Agricultural Product Storage and Processing Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, China
Yuwen Mu: Gansu Innovation Center of Fruit and Vegetable Storage and Processing, Lanzhou, China
Juan Song: Agricultural Product Storage and Processing Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, China
Juan Song: Gansu Innovation Center of Fruit and Vegetable Storage and Processing, Lanzhou, China

DOI: https://doi.org/10.3389/fpls.2022.887179
Journal volume & issue: Vol. 13

Abstract

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Sprouting is an irreversible deterioration of potato quality, which not only causes loss in their commercial value but also produces harmful toxins. As a popular disinfectant, ClO2 can inhibit the sprouting of potato tubers. Using transcriptomic and metabolomic approaches to understand the repressive mechanism of ClO2 in potato sprouting is yet to be reported. Sequencing the transcriptome and metabolome of potatoes treated with ClO2 in this study revealed a total of 3,119 differentially expressed genes, with 1,247 and 1,872 genes showing down- and upregulated expression, respectively. The majority of the downregulated genes were associated with plant hormone signal transduction, whereas upregulated differential genes were associated primarily with biological processes, such as phenylpropanoid biosynthesis and the mitogen-activated protein kinase (MAPK) signaling pathway. Metabonomic assays identified a total of 932 metabolites, with 33 and 52 metabolites being down- and upregulated, respectively. Downregulated metabolites were mostly alkaloids, amino acids, and their derivatives, whereas upregulated metabolites were composed mainly of flavonoids and coumarins. Integrated transcriptomic and metabolomic analyses showed that many different metabolites were regulated by several different genes, forming a complex regulatory network. These results provide new insights for understanding the mechanism of ClO2-mediated repression of potato sprouting.

Published in Frontiers in Plant Science

ISSN: 1664-462X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Agriculture: Plant culture
Website: https://www.frontiersin.org/journals/plant-science

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