Casein Kinase 1 Regulates Ethylene Synthesis by Phosphorylating and Promoting the Turnover of ACS5

Abstract

Summary: The casein kinase 1 (CK1) family participates in various cellular processes in eukaryotes, but CK1 function in higher plants remains largely unknown. Here, we characterize the function of Arabidopsis CK1 in the regulation of ethylene biosynthesis. Etiolated seedlings of a CK1.8-deficient mutant, ck1.8-1, showed characteristic ethylene-specific constitutive responses due to overaccumulation of ethylene. Biochemical and physiological studies showed that CK1.8 phosphorylates ACS5, a key enzyme of ethylene biosynthesis, at threonine 463 to promote its interaction with the E3 ubiquitin ligase Ethylene Overproduction 1 (ETO1). Deficiency of CK1.8 leads to the accumulation of ACS5, and transgenic plants harboring a dephosphorylation-mimic ACS5T463A showed constitutive ethylene responses, confirming the role of CK1.8 in regulating ACS5 stability by phosphorylation and demonstrating that CK1.8 is an important regulator of ethylene biosynthesis. CK1.8 expression is feedback regulated by ethylene. Our studies provide insight into the regulation of ACS5 and ethylene biosynthesis. : The role of casein kinase1 (CK1) in plants is largely unknown. Tan and Xue report that a CK1 isoform, CK1.8, acts as a negative regulator of ethylene biosynthesis in Arabidopsis by promoting, via phosphorylation, the degradation of an ethylene biosynthesis enzyme, ACS5. In Arabidopsis, loss of CK1.8 leads to phenotypes consistent with overproduction of ethylene, whereas in tomatoes overexpression delays fruit ripening, a result with potential agricultural implications.