Сучасна педіатрія: Україна (Apr 2024)
Single-nucleotide variant rs1800139 of the LRP1 gene as a factor in the development of obesity in children
Abstract
In the development of postnatal obesity and associated metabolic disorders, genetic features occupy a prominent place among various obesogenic factors. One of the genes that controls adipogenesis and determines the development of adipose tissue is the human low-density lipoprotein receptor-related protein 1 (LRP1) gene. The aim: study of the frequency of SNVs of the LRP1 gene in children with different phenotypes of obesity. Materials and methods. 253 obese children aged 6-18 years were examined. The main group (n=153) was made up of children with metabolically unhealthy obesity (MUO) according to IDEFICS 2014 criteria. The control group (n=100) was made up of children with metabolically healthy obesity (MHO). All children underwent a general clinical, immunobiochemical examination (Synevo, Ukraine). Whole-genome sequencing (CeGat, Germany) was performed in 31 children of the primary and 21 children of the control group. Static analysis: variational analysis, Wald analysis, calculation of χ2, Kramer's independence test, Spearman's correlation analysis, ROC analysis. Results. As a result of testing statistical hypotheses based on the conjugation table of features from 743 SNVs of 86 candidate genes studied, the greatest association with MUO was found in SNV rs1800139 LRP1 (V=0.44). In patients with MHO, the CC genotype was significantly less common (p<0.05) and the TT genotype of SNV rs1800139 of the LRP1 gene was more often registered than in the general human population (p<0.02). In patients with MUO, both homozygous genotypes of SNV rs1800139 of the LRP1 gene occurred significantly less frequently than in the general human population (p<0.05). Among patients with MUO, the TT genotype of SNV rs1800139 of the LRP1 gene occurred significantly less often compared to the group of patients with MHO (p<0.01). Conclusions. The СT genotype SNV rs1800139 of the LRP1 gene is highly associated with the presence of MUO and the development of dyslipidemia in children. The research was carried out in accordance with the principles of the Declaration of Helsinki. The research protocol was approved by the Local Ethics Committee of the institution mentioned in the work. Informed consent of parents or their guardians was obtained for conducting research. The authors declare no conflict of interest.
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