Fine-Tuning of <i>Arabidopsis thaliana</i> Response to Endophytic Colonization by <i>Gluconacetobacter diazotrophicus</i> PAL5 Revealed by Transcriptomic Analysis
Fabiano Silva Soares,
Ana Lídia Soares Rangel de Souza,
Suzane Ariádina de Souza,
Luciano de Souza Vespoli,
Vitor Batista Pinto,
Lucia Matiello,
Felipe Rodrigues da Silva,
Marcelo Menossi,
Gonçalo Apolinário de Souza Filho
Affiliations
Fabiano Silva Soares
Laboratório de Biotecnologia (Unidade de Biologia Integrativa), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro 28013-602, Brazil
Ana Lídia Soares Rangel de Souza
Laboratório de Biotecnologia (Unidade de Biologia Integrativa), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro 28013-602, Brazil
Suzane Ariádina de Souza
Laboratório de Biotecnologia (Unidade de Biologia Integrativa), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro 28013-602, Brazil
Luciano de Souza Vespoli
Laboratório de Biotecnologia (Unidade de Biologia Integrativa), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro 28013-602, Brazil
Vitor Batista Pinto
Laboratório de Biologia Celular e Tecidual, UENF, Campos dos Goytacazes, Rio de Janeiro 28013-602, Brazil
Lucia Matiello
Instituto de Biologia, Departamento de Genética, Evolução, Microbiologia e Imunologia, Universidade Estadual de Campinas (UNICAMP), Campinas, São Paulo 13083-970, Brazil
Felipe Rodrigues da Silva
Instituto de Biologia, Departamento de Genética, Evolução, Microbiologia e Imunologia, Universidade Estadual de Campinas (UNICAMP), Campinas, São Paulo 13083-970, Brazil
Marcelo Menossi
Instituto de Biologia, Departamento de Genética, Evolução, Microbiologia e Imunologia, Universidade Estadual de Campinas (UNICAMP), Campinas, São Paulo 13083-970, Brazil
Gonçalo Apolinário de Souza Filho
Laboratório de Biotecnologia (Unidade de Biologia Integrativa), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Campos dos Goytacazes, Rio de Janeiro 28013-602, Brazil
Gluconacetobacter diazotrophicus is a diazotrophic endophytic bacterium that promotes the growth and development of several plant species. However, the molecular mechanisms activated during plant response to this bacterium remain unclear. Here, we used the RNA-seq approach to understand better the effect of G. diazotrophicus PAL5 on the transcriptome of shoot and root tissues of Arabidopsis thaliana. G. diazotrophicus colonized A. thaliana roots and promoted growth, increasing leaf area and biomass. The transcriptomic analysis revealed several differentially expressed genes (DEGs) between inoculated and non-inoculated plants in the shoot and root tissues. A higher number of DEGs were up-regulated in roots compared to shoots. Genes up-regulated in both shoot and root tissues were associated with nitrogen metabolism, production of glucosinolates and flavonoids, receptor kinases, and transcription factors. In contrast, the main groups of down-regulated genes were associated with pathogenesis-related proteins and heat-shock proteins in both shoot and root tissues. Genes encoding enzymes involved in cell wall biogenesis and modification were down-regulated in shoots and up-regulated in roots. In contrast, genes associated with ROS detoxification were up-regulated in shoots and down-regulated in roots. These results highlight the fine-tuning of the transcriptional regulation of A. thaliana in response to colonization by G. diazotrophicus PAL5.
