Potensi Photodinamik Inaktivasi Staphylococcus aureus dan Vibrio cholerae dengan Endogen Photosensitizer pada Penyinaran Led Biru (430 ± 4) nm dan Merah (629 ± 6) nm

Abstract

Photodynamic Inactivation (PDI) is bacteria inactivation method with using light and bacteria porphyrin photosensitizer.The combination of light and photosensitizer with suitable spectrum can promote photosensitization process and then cause bacteriaphotodamage. This research was laboratory experiment, to analyze photodamage potency of Gram positive bacteria Staphylococcusaureus and Gram negative Vibrio cholera with combination of endogen photosensitizer and LED exposure (blue LED (430 ± 4) nmand red LED (629 ± 6) nm)) on Pulse Width Modulation (PWM) 75% and time duration 30 minutes. The viability of bacteria had beencounted after 48 hours incubation on temperature 37oC by using Total Plate Count (TPC). Result of this research showed that blueLED exposure (430 ± 4) nm had potency to decrease 70% Staphylococcus aureus and 50% Vibrio cholera bacteria colony formingunit. Red LED (629 ± 6) nm exposure decreased 22% dan 3% colony forming unit. So blue LED exposure had big potency to bacteriainactivate.

Keywords

• Photodynamic Inactivation
• porphyrin photosensitizer
• Staphylococcus aureus
• Vibrio cholerae
• Blue and red LED exposure
• Photodynamic Inactive
• Porphyrin photosensitizer