Antibiotic resistance: Detection of extended-spectrum-beta-lactamase in Enterobacteriaceae from garden eggs

Abstract

Background: The emphasis on sustainable good health through the consumption of a healthy diet has necessitated the consumption of fresh vegetables, which could harbour the presence of members of the Enterobacteriaceae, antibiotic resistance and extended-spectrum beta-lactamase (ESBL). As a result, this study investigated the presence of antibiotics resistance (AR) and ESBL in Enterobacteriaceae isolated from garden eggs. Methodology: One hundred (100) garden egg samples were randomly purchased from 10 different vendors into sterile bags. Samples were serially diluted and cultured on MacConkey agar for the isolation of Enterobacteriaceae, then characterised and identified. Antibiotic susceptibility test was carried out on isolates following the Kirby-Bauer disc diffusion method. Double disc synergy test (DDST) was used to detect ESBL production. Result: Forty-three isolates were identified to belong to the family Enterobacteriaceae with Klebsiella spp. being the most dominant specie (51.16%), Escherichia coli (30.23%), Salmonella (11.23%) and Enterobacter aerogenes (6.98%). Of all the isolates, (65.12%) were multi-drug resistant (MDR). The isolates showed highest frequency of resistance to erythromycin (90.7%), gentamicin (34.9%), sulfamethoxazole/trimethoprim (32.6%), ofloxacin (30.2%), ciprofloxacin (25.6%), imipenem (14%), ceftriaxone (11.6%) and nalidixic acid (0.0%). For ESBL production, 23(53.49%) were positive. The ESBL positive isolates (n=23) were Klebsiella spp. 14(60.87%) and Escherichia coli 9 (39.13%). No ESBL production was detected in Salmonella spp. and Enterobacter aerogenes isolates. Conclusion: This study detected the presence of AR and ESBL in Enterobacteriaceae from garden eggs. Consumption of garden eggs contaminated with these bacteria pose a potential problem of infection and spread of resistance in the environment through food.

Keywords

• enterobacteriaceae
• extended- spectrum beta-lactamase
• antibiotic
• resistance
• garden eggs