rL-RVG inhibits the proliferation and migration of human small cell lung cancer cell line NCI-H446

Abstract

Objective To investigate the effect and potential mechanism of recombinant Newcastle disease virus rL-RVG on proliferation and migration of human small cell lung cancer cell line NCI-H446. Methods NCI-H446 cells were divided into 3 groups: incubated with PBS, Newcastle disease virus(NDV) and rL-RVG, respectively. Immunofluorescence assay was used to detect the expression of RVG and NDV protein. MTT assay was used to determine the anti-proliferation of the virus. The cell migration was detected by scratch test and Transwell method. Immuno-fluorescence assay was used to detect the expression of E-cadherin and MMP2 proteins. Results After infection, rL-RVG stably expressed in NCI-H446 cell with high infection efficiency.The scratch distance in rL-RVG group was significantly shorter than that in NDV group and control group(P＜0.05). In the Transwell experiment, the number of cells crossing the compartment in the rL-RVG group were significantly less than that in the control group(P＜0.05). MMP2 protein expression was down-regulated in rL-RVG group, while E-cadherin protein expression was up-regulated(P＜0.05), compared with NDV group and control group. Conclusions rL-RVG could inhibit the proliferation of NCI-H446 cell with the potential mechanism of effects on cell migration through regulating E-cadherin and MMP2.

Keywords

• small cell lung cancer|newcastle disease virus|migration