Angiographic Imaging of an In Vivo Mouse Brain as a Guiding Star for Automatic Digital Refocusing in OCT

Abstract

A method allows the extraction of the recovery factor that maximizes the image contrast of OCT (optical coherence tomography) and/or OCTA (OCT angiography) of a living subject is proposed in this study. Due to the finite depth of focus in imaging optics, the volume OCT imaging suffers from blurriness in the lateral resolution. By utilizing the digital hologram method or angular spectrum method, the blurred image can be refocused. However, for in vivo OCT imaging, evaluation of the image focus is not easy, owing to the cloudy structure of the brain. In the proposed method, the blood flow signals were used as a guiding star to find the recovery factor. The propagation distance to a focal plane was automatically determined by evaluating the contrast of a cross-sectional OCTA image. The performance was examined though in vivo mouse brain OCT/OCTA imaging. The image singularity of the blood flow in OCTA was very effective at evaluating the contrast of the image.

Keywords

• digital focusing
• optical coherence tomography
• optical coherence tomography angiography
• brain tissues
• blood flow
• angular spectrum method
• vascular imaging