Production of crude enzyme from Trichoderma sp.

Abstract

Cellulose enzymes can degrade and hydrolyze the β-1,4 glycosidic bond in cellulose, resulting in various products, including glucose. This study aimed to find a species of Trichoderma and determine the optimal growth day for producing cellulase enzymes with the highest activity. Trichoderma is a fungus that can produce cellulase enzymes. The study was conducted to test the cellulase enzyme activity produced by a specific species of Trichoderma to identify the species with the highest activity level. A Trichoderma species that can increase and have the best activity in producing cellulase enzymes is required. The stages of cellulase enzyme production include mycelium growth on PDA media, enzyme sampling, activity testing using the 3,5-Dinitrosalicylic acid (DNS) method, and absorbance testing using a UV-Vis spectrophotometer. Based on the results of the production and activity testing of crude cellulase enzyme from Trichoderma selection at the Palm Oil Research Center, Bogor Unit, it was found that Trichoderma polysporum on day 3 of growth had the highest activity of 0.0583 U/mL.