Pathogens (Mar 2024)

Hunting for Answers: Assessing <i>Brucella</i> spp. Seroprevalence and Risks in Red Deer and Wild Boar in Central Portugal

  • Humberto Pires,
  • Luís Cardoso,
  • Ana Patrícia Lopes,
  • Maria da Conceição Fontes,
  • Sérgio Santos-Silva,
  • Manuela Matos,
  • Cristina Pintado,
  • Natália Roque,
  • Leonardo Filipe Fonseca,
  • Inês Morgado,
  • Ana Sofia Dias,
  • Luís Figueira,
  • Ana Cristina Matos,
  • João Rodrigo Mesquita,
  • Ana Cláudia Coelho

DOI
https://doi.org/10.3390/pathogens13030242
Journal volume & issue
Vol. 13, no. 3
p. 242

Abstract

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Between 2016 and 2023, a cross-sectional study was conducted in the central region of Portugal in order to better understand the epidemiology and public health risks resulting from the handling and consumption of game animals infected with Brucella spp. The seroprevalence and risk factors for Brucella spp. seropositivity were evaluated. Antibodies against Brucella spp. were determined using a commercial enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instructions. Results showed that in the 650 serum samples collected from red deer (n = 298) and wild boars (n = 352) in Portugal, 21.7% (n = 141; 95% CI: 18.6–25.1%) tested positive. Wild boar had a significantly higher prevalence (35.5%; 95% CI: 30.5–40.8%) than red deer (5.4%, 95% CI: 3.1–8.6%; p ≤ 0.001). Risk factors for seropositivity were investigated using multivariable logistic regression models. The odds of being seropositive was 8.39 (95% CI: 4.75–14.84; p ≤ 0.001) times higher in wild boar than in red deer. Correlations between sex, age, body condition, and seropositivity could not be observed. The higher seroprevalence in wild boar suggests that this species may primarily contribute to the Brucella spp. ecology in central Portugal.

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