Biosynthesis of copperoxide nanoparticles using Abies spectabilis plant extract and analyzing its antinociceptive and anti-inflammatory potency in various mice models

Abstract

Nanotechnology has shown rapid progress in various fields had made a remarkable change in the field of therapeutics too. The present study aims to synthesize and characterize the eco-friendly biocompatible copper oxide nanoparticles using Abies spectabilis plant extract (AS-CuONPs) and inspect its ameliorative potential against the different stimuli induced nociception and inflammatory reactions in mice. The formulated AS-CuONPs were characterized via the UV–vis spectroscopy, FT-IR, and TEM analysis. The nociception was induced by the method of acetic acid, glutamate, capsaicin, formalin, and thermal-induced techniques. The anti-inflammatory property of AS-CuONPs with carrageenan induced paw edema, peritoneal leukocyte infiltration, and carrageenan induced air pouch tests. Our result of UV–Visible spectroscopic analysis confirms the formation of AS-CuONPs and the characterization with FTIR and TEM proves the same. The result of various stimuli induced nociception models provide the evidence that formulated AS-CuONPs effectively inhibits the nociceptive responses in mice. Further the infiltration of leukocytes and inflammatory cytokines were drastically decreased by the AS-CuONPs treatment in the mice. The open field analysis confirms that the AS-CuONPs doesn’t impart any behavioral changes in mice. Over all our results confirms that AS-CuONPs is effective against different stimuli induced nociception and it act as a potent anti-inflammatory agent without rendering any side effects.

Keywords

• Copper oxide nanoparticles
• Green synthesis
• Abies spectabilis
• Antinociception
• Anti-inflammation