Effect of low molecular weight organic compound sodium 2-(2-hydroxyphenoxy)acetyl)-L-prolinate on rat gastric secretion stimulated by carbachol

Abstract

Тto investigate the effects of low molecular organic compound (LMOC) on gastric acid secretion (GAS), pepsin output and the activity of Н+,К+-ATPase in the conditions of carbachol stimulation. GAS stimulated by carbachol was measured by method of isolated perfused stomach, pepsin output was analyzed by colorimetric method for 120 minutes and the activity of Н+,К+-ATPase was assessed spectrophotometrically by the level of anorganic phosphate on 20 white laboratory rats. GAS, pepsin output and the Н+,К+-ATPase activity were decreased under the carbachol stimulation after injection of LMOC. LMOC decreases the gastric acid and pepsin secretion in terms of carbachol stimulation. The inhibition of Н+,К+-ATPase activity is considered as mechanism of antisecretory property of LMOC.

Keywords

• low molecular weight organic compound, stimulated secretion, hydrochloride acid, pepsin, Н+,К+-ATPase