PLoS ONE (Jan 2013)

VPPIPP and IPPVPP: two hexapeptides innovated to exert antihypertensive activity.

  • Fengyun Ding,
  • Bingjun Qian,
  • Xin Zhao,
  • Shanqi Shen,
  • Yun Deng,
  • Danfeng Wang,
  • Feng Zhang,
  • Zhongquan Sui,
  • Pu Jing

DOI
https://doi.org/10.1371/journal.pone.0062384
Journal volume & issue
Vol. 8, no. 4
p. e62384

Abstract

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In this study, two hexapeptides of IPPVPP and VPPIPP were innovated by using two commercial antihypertensive peptides IPP and VPP as two domains cis-linked and trans-linked, respectively. The IPPVPP and VPPIPP were chemically synthesized and evaluated for the antihypertensive activity in vitro/vivo. The in vitro ACE-inhibitory study showed that VPPIPP (34.71 ± 4.38%) has a significantly stronger activity than that of IPPVPP (13.17 ± 0.25%) at a treatment concentration of 10 µmol/L, but it was weaker than the commercial IPP (56.97 ± 2.40%) (P<0.05). However, VPPIPP, IPPVPP, and IPP lowered the systolic blood pressure by 21 ± 0.9%, 17.4 ± 1.3% and 17.5 ± 0.9%, respectively, in rats at 1.5 mg/kg body weight dosage. The result was consistent with the mRNA level of sarcoplasmic reticulum Ca(2+), Mg(2+) -ATPase Gene (SERCA 2a) in rat hearts. Additionally, VPPIPP and IPPVPP showed no negative impact on blood glycometabolism. The results suggested that the two hexapeptides could be potent bioactive peptides in functional foods for people with high blood pressure.