Identification of the New GmJAG1 Transcription Factor Binding Motifs Using DAP-Seq
Jinxing Wang,
Zigang Pu,
Weiyao Zhang,
Mengnan Qu,
Lusi Gao,
Wenjing Pan,
Yanan Sun,
Chunxu Fu,
Ling Zhang,
Mingkun Huang,
Yufang Hu
Affiliations
Jinxing Wang
Suihua Branch of the Heilongjiang Academy of Agricultural Sciences, Suihua 152052, China
Zigang Pu
Jiangxi Provincial Key Laboratory of Plant Germplasm Innovation and Genetic Improvement, Lushan Botanical Garden, Jiangxi Province and Chinese Academy of Sciences, Jiujiang 330022, China
Weiyao Zhang
Suihua Branch of the Heilongjiang Academy of Agricultural Sciences, Suihua 152052, China
Mengnan Qu
Suihua Branch of the Heilongjiang Academy of Agricultural Sciences, Suihua 152052, China
Lusi Gao
Suihua Branch of the Heilongjiang Academy of Agricultural Sciences, Suihua 152052, China
Wenjing Pan
Suihua Branch of the Heilongjiang Academy of Agricultural Sciences, Suihua 152052, China
Yanan Sun
Suihua Branch of the Heilongjiang Academy of Agricultural Sciences, Suihua 152052, China
Chunxu Fu
Suihua Branch of the Heilongjiang Academy of Agricultural Sciences, Suihua 152052, China
Ling Zhang
Jiangxi Provincial Key Laboratory of Plant Germplasm Innovation and Genetic Improvement, Lushan Botanical Garden, Jiangxi Province and Chinese Academy of Sciences, Jiujiang 330022, China
Mingkun Huang
Jiangxi Provincial Key Laboratory of Plant Germplasm Innovation and Genetic Improvement, Lushan Botanical Garden, Jiangxi Province and Chinese Academy of Sciences, Jiujiang 330022, China
Yufang Hu
Jiangxi Provincial Key Laboratory of Plant Germplasm Innovation and Genetic Improvement, Lushan Botanical Garden, Jiangxi Province and Chinese Academy of Sciences, Jiujiang 330022, China
Interaction between transcription factors (TFs) and motifs is essential for gene regulation and the subsequent phenotype formation. Soybean (Glycine max) JAGGEED 1 (GmJAG1) is a key TF that controls leaf shape, seed number and flower size. To understand the GmJAG1 binding motifs, in this study, we performed the GmJAG1 DNA affinity purification sequencing (DAP-seq) experiment, which is a powerful tool for the de novo motif prediction method. Two new significant GmJAG1 binding motifs were predicted and the EMSA experiments further verified the ability of GmJAG1 bound to these motifs. The potential binding sites in the downstream gene promoter were identified through motif scanning and a potential regulatory network mediated by GmJAG1 was constructed. These results served as important genomic resources for further understanding the regulatory mechanism of GmJAG1.
