Amplicon sequencing based profiling of bacterial diversity from Krossfjorden, Arctic
Bhavya Kachiprath,
Jayesh Puthumana,
Jayanath Gopi,
Solly Solomon,
K.P. Krishnan,
Rosamma Philip
Affiliations
Bhavya Kachiprath
Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Kochi 682016, Kerala, India
Jayesh Puthumana
National Centre for Aquatic Animal Health, Cochin University of Science and Technology, Fine Arts Avenue, Kochi 682016, Kerala, India
Jayanath Gopi
Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Kochi 682016, Kerala, India
Solly Solomon
Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Kochi 682016, Kerala, India; Fishery Survey of India, Mormugao Zonal Base, Vasco da Gama, Goa, India
K.P. Krishnan
National Centre for Polar and Ocean Research, Ministry of Earth Sciences, Government of India, Goa, India
Rosamma Philip
Department of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology, Kochi 682016, Kerala, India; Corresponding author.
In this study, Illumina Miseq sequencing of 16S rRNA gene amplicon was performed on sediments collected from Krossfjorden, Arctic for analyzing the bacterial community structure. Metagenome contained 15,936 sequences with 5,809,491 bp size and 53% G+C content. Metagenome sequence information are now available at NCBI under the Sequence Read Archive (SRA) database with accession no. SRP159159. Taxonomic hits distribution from MG-RAST analysis revealed the dominance of Alpha- and Gamma-subdivisions of Proteobacteria (88.89%) along with Bacteriodetes (8.89%) and Firmicutes (2.22%). Predominant species were Alteromonadales bacterium TW-7 (24%), Pseudoalteromonas haloplanktis (20%) and Pseudoalteromonas spp. SM9913 (18%). MG-RAST assisted analysis also detected the presence of a variety of marine taxa like Bacteriodes, Pseudovibrio, Marinobacter, Idiomarina, Teredinibacter, etc. which take part in key ecological functions and biogeochemical activities of Arctic fjord ecosystems. Keywords: Arctic, Bacterial diversity, Metagenome, Amplicon sequencing, Illumina
