EPJ Web of Conferences (Mar 2013)

Pulse-shaping-based two-photon FRET microscopy

  • Swanson J. A.,
  • Straight S. W.,
  • Cai D.,
  • Brenner M. H.,
  • Ogilvie J. P.

DOI
https://doi.org/10.1051/epjconf/20134111009
Journal volume & issue
Vol. 41
p. 11009

Abstract

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We present quantitative pulse-shaping-based two-photon fluorescence resonance energy transfer microscopy. We tailor the spectral phase of the excitation pulses to achieve selective excitation of donor and acceptor, demonstrating the method in live cells.