Cytoprotective Activity of <i>p</i>-Terphenyl Polyketides and Flavuside B from Marine-Derived Fungi against Oxidative Stress in Neuro-2a Cells
Ekaterina A. Yurchenko,
Ekaterina S. Menchinskaya,
Evgeny A. Pislyagin,
Ekaterina A. Chingizova,
Elena V. Girich,
Anton N. Yurchenko,
Dmitry L. Aminin,
Valery V. Mikhailov
Affiliations
Ekaterina A. Yurchenko
Laboratory of Bioassays and Mechanism of Action of Biologically Active Substances, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Prosp. 100 Let Vladivostoku 159, 690022 Vladivostok, Russia
Ekaterina S. Menchinskaya
Laboratory of Bioassays and Mechanism of Action of Biologically Active Substances, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Prosp. 100 Let Vladivostoku 159, 690022 Vladivostok, Russia
Evgeny A. Pislyagin
Laboratory of Bioassays and Mechanism of Action of Biologically Active Substances, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Prosp. 100 Let Vladivostoku 159, 690022 Vladivostok, Russia
Ekaterina A. Chingizova
Laboratory of Bioassays and Mechanism of Action of Biologically Active Substances, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Prosp. 100 Let Vladivostoku 159, 690022 Vladivostok, Russia
Elena V. Girich
Laboratory of Chemistry of Microbial Metabolites, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Prosp. 100 Let Vladivostoku 159, 690022 Vladivostok, Russia
Anton N. Yurchenko
Laboratory of Chemistry of Microbial Metabolites, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Prosp. 100 Let Vladivostoku 159, 690022 Vladivostok, Russia
Dmitry L. Aminin
Laboratory of Bioassays and Mechanism of Action of Biologically Active Substances, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Prosp. 100 Let Vladivostoku 159, 690022 Vladivostok, Russia
Valery V. Mikhailov
Laboratory of Microbiology, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Prosp. 100 Let Vladivostoku 159, 690022 Vladivostok, Russia
The influence of p-terphenyl polyketides 1–3 from Aspergillus candidus KMM 4676 and cerebroside flavuside B (4) from Penicillium islandicum (=Talaromyces islandicus) against the effect of neurotoxins, rotenone and paraquat, on Neuro-2a cell viability by MTT and LDH release assays and intracellular ROS level, as well as DPPH radical scavenging activity, was investigated. Pre-incubation with compounds significantly diminished the ROS level in rotenone- and paraquat-treated cells. It was shown that the investigated polyketides 1–3 significantly increased the viability of rotenone- and paraquat-treated cells in two of the used assays but they affected only the viability of paraquat-treated cells in the LDH release assay. Flavuside B statistically increased the viability of paraquat-treated cells in both MTT and LDH release assays, however, it increased the viability of rotenone-treated cells in the LDH release assay. Structure–activity relationships for p-terphenyl derivatives, as well as possible mechanisms of cytoprotective action of all studied compounds, were discussed.
