Nature Communications (Apr 2022)
Development of NanoLuc-targeting protein degraders and a universal reporter system to benchmark tag-targeted degradation platforms
- Christoph Grohmann,
- Charlene M. Magtoto,
- Joel R. Walker,
- Ngee Kiat Chua,
- Anna Gabrielyan,
- Mary Hall,
- Simon A. Cobbold,
- Stephen Mieruszynski,
- Martin Brzozowski,
- Daniel S. Simpson,
- Hao Dong,
- Bridget Dorizzi,
- Annette V. Jacobsen,
- Emma Morrish,
- Natasha Silke,
- James M. Murphy,
- Joan K. Heath,
- Andrea Testa,
- Chiara Maniaci,
- Alessio Ciulli,
- Guillaume Lessene,
- John Silke,
- Rebecca Feltham
Affiliations
- Christoph Grohmann
- The Walter and Eliza Hall Institute for Medical Research
- Charlene M. Magtoto
- The Walter and Eliza Hall Institute for Medical Research
- Joel R. Walker
- Promega Biosciences LLC
- Ngee Kiat Chua
- The Walter and Eliza Hall Institute for Medical Research
- Anna Gabrielyan
- The Walter and Eliza Hall Institute for Medical Research
- Mary Hall
- Promega Biosciences LLC
- Simon A. Cobbold
- The Walter and Eliza Hall Institute for Medical Research
- Stephen Mieruszynski
- The Walter and Eliza Hall Institute for Medical Research
- Martin Brzozowski
- The Walter and Eliza Hall Institute for Medical Research
- Daniel S. Simpson
- The Walter and Eliza Hall Institute for Medical Research
- Hao Dong
- The Walter and Eliza Hall Institute for Medical Research
- Bridget Dorizzi
- The Walter and Eliza Hall Institute for Medical Research
- Annette V. Jacobsen
- The Walter and Eliza Hall Institute for Medical Research
- Emma Morrish
- The Walter and Eliza Hall Institute for Medical Research
- Natasha Silke
- The Walter and Eliza Hall Institute for Medical Research
- James M. Murphy
- The Walter and Eliza Hall Institute for Medical Research
- Joan K. Heath
- The Walter and Eliza Hall Institute for Medical Research
- Andrea Testa
- Amphista Therapeutics Ltd
- Chiara Maniaci
- Chemistry School of Natural and Environmental Sciences, Bedson Building, Newcastle University Edwards Walk
- Alessio Ciulli
- Division of Biological Chemistry and Drug Discovery, School of Life Sciences, University of Dundee
- Guillaume Lessene
- The Walter and Eliza Hall Institute for Medical Research
- John Silke
- The Walter and Eliza Hall Institute for Medical Research
- Rebecca Feltham
- The Walter and Eliza Hall Institute for Medical Research
- DOI
- https://doi.org/10.1038/s41467-022-29670-1
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 13
Abstract
tag-Targeted Protein Degrader (tTPD) systems are powerful tools for preclinical target validation. Here the authors extend the tTPD platform by developing NanoTACs that degrade NanoLuc tagged substrates and benchmark each tTPD system using an interchangeable tag reporter system.
