Large-Scale Profiling on lncRNAs in Human Platelets: Correlation with Platelet Reactivity
Yeying Sun,
Rongrong Liu,
Xiangwen Xia,
Luchuan Xing,
Jing Jiang,
Weihua Bian,
Wendy Zhang,
Chunhua Wang,
Chunxiang Zhang
Affiliations
Yeying Sun
US-China Institute for Translational Medicine, College of Pharmacy, Binzhou Medical University, Yantai 264000, China
Rongrong Liu
US-China Institute for Translational Medicine, College of Pharmacy, Binzhou Medical University, Yantai 264000, China
Xiangwen Xia
US-China Institute for Translational Medicine, College of Pharmacy, Binzhou Medical University, Yantai 264000, China
Luchuan Xing
US-China Institute for Translational Medicine, College of Pharmacy, Binzhou Medical University, Yantai 264000, China
Jing Jiang
US-China Institute for Translational Medicine, College of Pharmacy, Binzhou Medical University, Yantai 264000, China
Weihua Bian
US-China Institute for Translational Medicine, College of Pharmacy, Binzhou Medical University, Yantai 264000, China
Wendy Zhang
Department of Biomedical Engineering, School of Medicine, The University of Alabama at Birmingham, Birmingham, AL 35233, USA
Chunhua Wang
US-China Institute for Translational Medicine, College of Pharmacy, Binzhou Medical University, Yantai 264000, China
Chunxiang Zhang
Department of Cardiology, The Affiliated Hospital of Southwest Medical University, Key Laboratory of Medical Electrophysiology of Ministry of Education, Institute of Cardiovascular Research, Nucleic Acid Medicine of Luzhou Key Laboratory, Metabolic Vascular Disease Key Laboratory of Sichuan Province, Southwest Medical University, Luzhou 646000, China
Recently, long noncoding RNAs (lncRNAs) have been key regulators for both mRNAs and proteins in nucleated cells. However, the expression profiles of lncRNAs in non-nucleated cells such as platelets are currently unclear. In this study, we determined the expression profiles of lncRNAs in human platelets. We found that 6109 lncRNAs were expressed in human platelets. Interestingly, 338 lncRNAs were differentially expressed in hyperreactive and hyporeactive platelets. Bioinformatics’ analysis revealed that these aberrantly expressed lncRNAs might be related to platelet activity and other platelet functions. To provide a proof of concept, we measured the expression levels of PARLncRNA-1, a down-regulated lncRNA of hyperreactive platelets, in platelets from 12 patients with acute myocardial infarction and their controls. We found that the lncRNA was also significantly down-regulated in platelets from patients, which was partially reversed by treatment with aspirin a known antiplatelet drug. LncRNAs may represent a novel class of modulators for platelet functions.
