Using grain protein electrophoresis to control the genetic integrity of spring bread wheat accessions from the VIR collection of plant genetic resources

Abstract

Background. VIR's Department of Wheat holds the collections of viable accessions and its originals. The process of reproduction and restoration of germination ability in wheat collections requires monitoring of varietal purity and integrity. For this purpose, botanical varieties that describe sets of morphological characteristics of the spike and grain are used. However, sometimes it is necessary to check the conformity of a variety with its original accession. In this case, the gliadin electrophoretic banding patterns are reliable markers in identifying cultivars and biotypes of wheat.Materials and methods. The study included 7 accessions of spring bread wheat: 3 for the cultivar 'Rubin' and 4 for the cultivar 'Novinka'. Identification of accessions by electrophoresis of gliadin storage protein was carried out according to the generally accepted method.Results and conclusions. Comparison of gliadin electrophoretic banding patterns of the varieties from the VIR collection of plant genetic resources with analogous accessions from other genebanks of the world resulted in the recovery of the lost varieties 'Rubin' (Sweden) and 'Novinka' (Russia).

Keywords

• spring bread wheat
• cultivar
• gliadin electrophoretic banding pattern