Development of a Reference Transcriptome and Identification of Differentially Expressed Genes Linked to Salt Stress in Salt Marsh Grass (<i>Sporobolus alterniflorus</i>) along Delaware Coastal Regions

Abstract

Salt marsh grass (Sporobolus alterniflorus) plays a crucial role in Delaware coastal regions by serving as a physical barrier between land and water along the inland bays and beaches. This vegetation helps to stabilize the shoreline and prevent erosion, protecting the land from the powerful forces of the waves and tides. In addition to providing a physical barrier, salt marsh grass is responsible for filtering nutrients in the water, offering an environment for aquatic species and presenting a focal point of study for high salt tolerance in plants. As seawater concentrations vary along the Delaware coast from low to medium to high salinity, our study seeks to identify the impact of salt tolerance in marsh grass and to identify genes associated with salt tolerance levels. We developed more than 211,000 next-generation-sequencing (Illumina) transcriptomic reads to create a reference transcriptome from low-, medium-, and high-salinity marsh grass leaf samples collected from the Delaware coastline. Contiguous sequences were annotated based on a homology search using BLASTX against rice (Oryza sativa), foxtail millet (Setaria italica), and non-redundant species within the Viridiplantae database. Additionally, we identified differentially expressed genes related to salinity stress as candidates for salt stress qPCR analysis. The data generated from this study may help to elucidate the genetic signatures and physiological responses of plants to salinity stress, thereby offering valuable insight into the use of innovative approaches for gene expression studies in crops that are less salt tolerant.

Keywords

• transcriptome
• next-generation sequencing
• marsh grass