An inducible iFUCCI reporter cell line for tracking cell cycle dynamics in human pluripotent stem cells and their differentiated progeny

Merve Ay; Desi Veleva; Mohammad Chowdhury; Sonja Drljaca; Thais Sobanski; Andrew B.J. Prowse; Dmitry A. Ovchinnikov

Stem Cell Research (Dec 2024)

An inducible iFUCCI reporter cell line for tracking cell cycle dynamics in human pluripotent stem cells and their differentiated progeny

Merve Ay,
Desi Veleva,
Mohammad Chowdhury,
Sonja Drljaca,
Thais Sobanski,
Andrew B.J. Prowse,
Dmitry A. Ovchinnikov

Affiliations

Merve Ay: StemCore, The Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia 4072, QLD, Australia
Desi Veleva: StemCore, The Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia 4072, QLD, Australia; Corresponding authors at: StemCore, The Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia 4072, QLD, Australia (D.A. Ovchinnikov).
Mohammad Chowdhury: StemCore, The Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia 4072, QLD, Australia
Sonja Drljaca: StemCore, The Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia 4072, QLD, Australia
Thais Sobanski: StemCore, The Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia 4072, QLD, Australia
Andrew B.J. Prowse: StemCore, The Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia 4072, QLD, Australia
Dmitry A. Ovchinnikov: StemCore, The Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia 4072, QLD, Australia; The Florey, The University of Melbourne, 30 Royal Parade, Melbourne, VIC 3010, Australia; Corresponding authors at: StemCore, The Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia 4072, QLD, Australia (D.A. Ovchinnikov).

Journal volume & issue: Vol. 81
p. 103614

Abstract

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An ability to monitor cell cycle progression in real time has numerous applications in pluripotent stem cell-based models and therapeutics development. Fluorescence Ubiquitination-based Cell Cycle Indicator (FUCCI) systems enable live monitoring of the cell cycle in stem cells and their differentiated progenies in a non-invasive manner. We describe the generation and characterisation of a cell line with a doxycycline-inducible reporter system, iFUCCI, in the human embryonic stem cell (hESC) line MEL-1. MEL-1 iFUCCI hESCs exhibited a normal karyotype, expressed markers of the undifferentiated state, and demonstrated expected differentiation potential by giving rise to cell populations from all three germ layers.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

About the journal