Golgi satellites are essential for polysialylation of NCAM and expression of LTP at distal synapses
Maria Andres-Alonso,
Maximilian Borgmeyer,
Hadi Mirzapourdelavar,
Jakob Lormann,
Kim Klein,
Michaela Schweizer,
Sabine Hoffmeister-Ullerich,
Anja M. Oelschlegel,
Alexander Dityatev,
Michael R. Kreutz
Affiliations
Maria Andres-Alonso
Leibniz Group “Dendritic Organelles and Synaptic Function,” Center for Molecular Neurobiology (ZMNH), University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany; RG Neuroplasticity, Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany; Corresponding author
Maximilian Borgmeyer
Leibniz Group “Dendritic Organelles and Synaptic Function,” Center for Molecular Neurobiology (ZMNH), University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany; RG Neuroplasticity, Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany
Hadi Mirzapourdelavar
German Center for Neurodegenerative Diseases (DZNE), 39120 Magdeburg, Germany
Jakob Lormann
Leibniz Group “Dendritic Organelles and Synaptic Function,” Center for Molecular Neurobiology (ZMNH), University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany; RG Neuroplasticity, Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany
Kim Klein
Leibniz Group “Dendritic Organelles and Synaptic Function,” Center for Molecular Neurobiology (ZMNH), University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany
Michaela Schweizer
Core Facility Morphology und Electron Microscopy, Center for Molecular Neurobiology (ZMNH), University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany
Sabine Hoffmeister-Ullerich
Core Facility Bioanalytik, Center for Molecular Neurobiology (ZMNH), University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany
Anja M. Oelschlegel
RG Neuroplasticity, Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany
Alexander Dityatev
German Center for Neurodegenerative Diseases (DZNE), 39120 Magdeburg, Germany; Center for Behavioral Brain Sciences, Otto von Guericke University, 39120 Magdeburg, Germany; Medical Faculty, Otto von Guericke University, 39120 Magdeburg, Germany
Michael R. Kreutz
Leibniz Group “Dendritic Organelles and Synaptic Function,” Center for Molecular Neurobiology (ZMNH), University Medical Center Hamburg-Eppendorf, 20251 Hamburg, Germany; RG Neuroplasticity, Leibniz Institute for Neurobiology, 39118 Magdeburg, Germany; German Center for Neurodegenerative Diseases (DZNE), 39120 Magdeburg, Germany; Center for Behavioral Brain Sciences, Otto von Guericke University, 39120 Magdeburg, Germany; Corresponding author
Summary: The complex cytoarchitecture of neurons poses significant challenges for the maturation of synaptic membrane proteins. It is currently unclear whether locally secreted synaptic proteins bypass the Golgi or whether they traffic through Golgi satellites (GSs). Here, we create a transgenic GS reporter mouse line and show that GSs are widely distributed along dendrites and are capable of mature glycosylation, in particular sialylation. We find that polysialylation of locally secreted NCAM takes place at GSs. Accordingly, in mice lacking a component of trans-Golgi network-to-plasma membrane trafficking, we find fewer GSs and significantly reduced PSA-NCAM levels in distal dendrites of CA1 neurons that receive input from the temporoammonic pathway. Induction of long-term potentiation at those, but not more proximal, synapses is severely impaired. We conclude that GSs serve the need for local mature glycosylation of synaptic membrane proteins in distal dendrites and thereby contribute to rapid changes in synaptic strength.
