Neuroprotective impact of Ximenia americana aqueous bark extract on Diazepam-induced memory impairment in mice via its antioxidant potential

Abstract

In traditional medicine, Ximenia americana (XA) is used to treat mental disorders, and headaches. The current study aimed to show the preventive and biochemical impacts of XA aqueou’s extract on diazepam-induced amnesia. Mice were randomized as follows: distilled water (10 mL/kg); diazepam (3 mg/kg); piracetam (PIR) (150 mg/kg); and XA experimental groups (25, 50 and 75 mg/kg). Mice were then treated in groups, 14 straight days. Radial arm maze (RAM) and T-maze were employed to assess different behaviours 30 min after each treatment. After the test was completed, the brains were isolated for histological and biochemical examinations. The results obtained showed that XA extract seriously (p < 0.001) reversed mistakes in working remembrance in the radial arm maze test contrasted to the normal control factions. In the T-maze test, pretreatment of mice with XA extract seriously (p < 0.001) expanded the time spent in the preferred arm when contrasted to the DZP-only treated faction. The XA-treated DZP groups showed subsequent (p < 0.001) improvement in catalase (CAT) and reduced glutathione (GHS). A diminish in malondialdehyde (MDA) level was observed in brain homogenates of mice treated with the extract contrasted with the DZP- group. These few results regarding the neuroprotective and antioxidant effects of XA extract at least partially demonstrate its empirical use in the treatment of certain pathologies.

Keywords

• amnesia
• antioxidant
• diazepam
• neuroprotective
• Ximenia americana