Genome Biology (Sep 2019)
Comparative genomic analysis of six Glossina genomes, vectors of African trypanosomes
- Geoffrey M. Attardo,
- Adly M. M. Abd-Alla,
- Alvaro Acosta-Serrano,
- James E. Allen,
- Rosemary Bateta,
- Joshua B. Benoit,
- Kostas Bourtzis,
- Jelle Caers,
- Guy Caljon,
- Mikkel B. Christensen,
- David W. Farrow,
- Markus Friedrich,
- Aurélie Hua-Van,
- Emily C. Jennings,
- Denis M. Larkin,
- Daniel Lawson,
- Michael J. Lehane,
- Vasileios P. Lenis,
- Ernesto Lowy-Gallego,
- Rosaline W. Macharia,
- Anna R. Malacrida,
- Heather G. Marco,
- Daniel Masiga,
- Gareth L. Maslen,
- Irina Matetovici,
- Richard P. Meisel,
- Irene Meki,
- Veronika Michalkova,
- Wolfgang J. Miller,
- Patrick Minx,
- Paul O. Mireji,
- Lino Ometto,
- Andrew G. Parker,
- Rita Rio,
- Clair Rose,
- Andrew J. Rosendale,
- Omar Rota-Stabelli,
- Grazia Savini,
- Liliane Schoofs,
- Francesca Scolari,
- Martin T. Swain,
- Peter Takáč,
- Chad Tomlinson,
- George Tsiamis,
- Jan Van Den Abbeele,
- Aurelien Vigneron,
- Jingwen Wang,
- Wesley C. Warren,
- Robert M. Waterhouse,
- Matthew T. Weirauch,
- Brian L. Weiss,
- Richard K. Wilson,
- Xin Zhao,
- Serap Aksoy
Affiliations
- Geoffrey M. Attardo
- Department of Entomology and Nematology, University of California, Davis
- Adly M. M. Abd-Alla
- Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food & Agriculture
- Alvaro Acosta-Serrano
- Department of Vector Biology, Liverpool School of Tropical Medicine
- James E. Allen
- VectorBase, European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI)
- Rosemary Bateta
- Department of Biochemistry, Biotechnology Research Institute - Kenya Agricultural and Livestock Research Organization
- Joshua B. Benoit
- Department of Biological Sciences, University of Cincinnati
- Kostas Bourtzis
- Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food & Agriculture
- Jelle Caers
- Department of Biology - Functional Genomics and Proteomics Group, KU Leuven
- Guy Caljon
- Laboratory of Microbiology, Parasitology and Hygiene, University of Antwerp
- Mikkel B. Christensen
- VectorBase, European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI)
- David W. Farrow
- Department of Biological Sciences, University of Cincinnati
- Markus Friedrich
- Department of Biological Sciences, Wayne State University
- Aurélie Hua-Van
- Laboratoire Evolution, Genomes, Comportement, Ecologie, CNRS, IRD, Univ. Paris-Sud, Université Paris-Saclay
- Emily C. Jennings
- Department of Biological Sciences, University of Cincinnati
- Denis M. Larkin
- Department of Comparative Biomedical Sciences, Royal Veterinary College
- Daniel Lawson
- Department of Life Sciences, Imperial College London
- Michael J. Lehane
- Department of Vector Biology, Liverpool School of Tropical Medicine
- Vasileios P. Lenis
- Schools of Medicine and Dentistry, University of Plymouth
- Ernesto Lowy-Gallego
- VectorBase, European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI)
- Rosaline W. Macharia
- Molecular Biology and Bioinformatics Unit, International Center for Insect Physiology and Ecology
- Anna R. Malacrida
- Department of Biology and Biotechnology, University of Pavia
- Heather G. Marco
- Department of Biological Sciences, University of Cape Town
- Daniel Masiga
- Molecular Biology and Bioinformatics Unit, International Center for Insect Physiology and Ecology
- Gareth L. Maslen
- VectorBase, European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI)
- Irina Matetovici
- Biomedical Sciences, Institute of Tropical Medicine
- Richard P. Meisel
- Department of Biology and Biochemistry, University of Houston
- Irene Meki
- Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food & Agriculture
- Veronika Michalkova
- Department of Biological Sciences, Florida International University
- Wolfgang J. Miller
- Department of Cell and Developmental Biology, Medical University of Vienna
- Patrick Minx
- McDonnell Genome Institute, Washington University School of Medicine
- Paul O. Mireji
- Department of Biochemistry, Biotechnology Research Institute - Kenya Agricultural and Livestock Research Organization
- Lino Ometto
- Department of Sustainable Ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund Mach
- Andrew G. Parker
- Insect Pest Control Laboratory, Joint FAO/IAEA Division of Nuclear Techniques in Food & Agriculture
- Rita Rio
- Department of Biology, West Virginia University
- Clair Rose
- Department of Vector Biology, Liverpool School of Tropical Medicine
- Andrew J. Rosendale
- Department of Biology, Mount St. Joseph University
- Omar Rota-Stabelli
- Department of Sustainable Ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund Mach
- Grazia Savini
- Department of Biology and Biotechnology, University of Pavia
- Liliane Schoofs
- Department of Biology - Functional Genomics and Proteomics Group, KU Leuven
- Francesca Scolari
- Department of Biology and Biotechnology, University of Pavia
- Martin T. Swain
- Institute of Biological, Environmental and Rural Sciences, Aberystwyth University
- Peter Takáč
- Department of Animal Systematics, Ústav zoológie SAV; Scientica, Ltd
- Chad Tomlinson
- McDonnell Genome Institute, Washington University School of Medicine
- George Tsiamis
- Department of Environmental and Natural Resources Management, University of Patras
- Jan Van Den Abbeele
- Biomedical Sciences, Institute of Tropical Medicine
- Aurelien Vigneron
- Department of Epidemiology of Microbial Diseases, Yale School of Public Health
- Jingwen Wang
- School of Life Sciences, Fudan University
- Wesley C. Warren
- McDonnell Genome Institute, Washington University School of Medicine
- Robert M. Waterhouse
- Department of Ecology & Evolution, Swiss Institute of Bioinformatics, University of Lausanne
- Matthew T. Weirauch
- Center for Autoimmune Genomics and Etiology and Divisions of Biomedical Informatics and Developmental Biology, Cincinnati Children’s Hospital Medical Center
- Brian L. Weiss
- Department of Epidemiology of Microbial Diseases, Yale School of Public Health
- Richard K. Wilson
- McDonnell Genome Institute, Washington University School of Medicine
- Xin Zhao
- CAS Center for Influenza Research and Early-warning (CASCIRE), Chinese Academy of Sciences
- Serap Aksoy
- Department of Epidemiology of Microbial Diseases, Yale School of Public Health
- DOI
- https://doi.org/10.1186/s13059-019-1768-2
- Journal volume & issue
-
Vol. 20,
no. 1
pp. 1 – 31
Abstract
Abstract Background Tsetse flies (Glossina sp.) are the vectors of human and animal trypanosomiasis throughout sub-Saharan Africa. Tsetse flies are distinguished from other Diptera by unique adaptations, including lactation and the birthing of live young (obligate viviparity), a vertebrate blood-specific diet by both sexes, and obligate bacterial symbiosis. This work describes the comparative analysis of six Glossina genomes representing three sub-genera: Morsitans (G. morsitans morsitans, G. pallidipes, G. austeni), Palpalis (G. palpalis, G. fuscipes), and Fusca (G. brevipalpis) which represent different habitats, host preferences, and vectorial capacity. Results Genomic analyses validate established evolutionary relationships and sub-genera. Syntenic analysis of Glossina relative to Drosophila melanogaster shows reduced structural conservation across the sex-linked X chromosome. Sex-linked scaffolds show increased rates of female-specific gene expression and lower evolutionary rates relative to autosome associated genes. Tsetse-specific genes are enriched in protease, odorant-binding, and helicase activities. Lactation-associated genes are conserved across all Glossina species while male seminal proteins are rapidly evolving. Olfactory and gustatory genes are reduced across the genus relative to other insects. Vision-associated Rhodopsin genes show conservation of motion detection/tracking functions and variance in the Rhodopsin detecting colors in the blue wavelength ranges. Conclusions Expanded genomic discoveries reveal the genetics underlying Glossina biology and provide a rich body of knowledge for basic science and disease control. They also provide insight into the evolutionary biology underlying novel adaptations and are relevant to applied aspects of vector control such as trap design and discovery of novel pest and disease control strategies.
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