A Key Role of CD8+ T Cells in Controlling of Tuberculosis Infection
Igor Kudryavtsev,
Yulia Zinchenko,
Maria Serebriakova,
Tatiana Akisheva,
Artem Rubinstein,
Andrei Savchenko,
Alexandr Borisov,
Vasilij Belenjuk,
Anna Malkova,
Piotr Yablonskiy,
Dmitry Kudlay,
Anna Starshinova
Affiliations
Igor Kudryavtsev
Institution of Experimental Medicine, Department of Immunology, 197376 St-Petersburg, Russia
Yulia Zinchenko
Research Institute of Phthisiopulmonology, 191036 St-Petersburg, Russia
Maria Serebriakova
Institution of Experimental Medicine, Department of Immunology, 197376 St-Petersburg, Russia
Tatiana Akisheva
Institution of Experimental Medicine, Department of Immunology, 197376 St-Petersburg, Russia
Artem Rubinstein
Institution of Experimental Medicine, Department of Immunology, 197376 St-Petersburg, Russia
Andrei Savchenko
Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North, 660022 Krasnoyarsk, Russia
Alexandr Borisov
Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North, 660022 Krasnoyarsk, Russia
Vasilij Belenjuk
Federal Research Center «Krasnoyarsk Science Center» of the Siberian Branch of the Russian Academy of Sciences, Scientific Research Institute of Medical Problems of the North, 660022 Krasnoyarsk, Russia
Anna Malkova
Department of Molecular Biology, Faculty of Natural Sciences, Ariel University, Ariel 40700, Israel
Piotr Yablonskiy
Research Institute of Phthisiopulmonology, 191036 St-Petersburg, Russia
Dmitry Kudlay
Department of Pharmacology, I.M. Sechenov First Moscow State Medical University, 119991 Moscow, Russia
Anna Starshinova
Almazov National Medical Research Centre, 197341 St-Petersburg, Russia
The main role in the control of tuberculosis infection is played by macrophages and Th1 and CD8+ T cells. The study aimed to identify the most diagnostically significant CD8+ T cell subsets in tuberculosis patients. Methods: Peripheral blood samples from patients with clinical, radiological, and bacteriologically confirmed pulmonary tuberculosis (TB, n = 32) and healthy subjects (HC, n = 31) were collected and analyzed using 10-color flow cytometry. Results: The frequency of the EM4 CD3+CD8+ cells was reduced in the peripheral blood of patients with pulmonary tuberculosis, while the relative and absolute number of EM1 CD3+CD8+ cells increased compared to the control group. CD57 expression was reduced in patients with pulmonary tuberculosis on EM1, EM2, and pE1 CD3+CD8+ cells, whereas the EM3 cells had a high level of CD57 expression. The relative and absolute number of Tc2 (CCR6–CXCR3–) cells in peripheral blood in patients with pulmonary tuberculosis was increased, while the frequency of Tc1 (CCR6–CXCR3+) was decreased, compared to healthy donors. Conclusions: Patients with pulmonary tuberculosis have an abnormal CD3+CD8+ cell profile and demonstrate their impaired maturation and functional activity.
