The interaction properties of the seeds have to be calibrated to simulate the realistic behavior of the seed bulk. Here, a simple and accurate calibration method of DEM interaction properties of seeds with adequate equipment to simulate each seed’s behavior remains a challenge. In this research, the rotary drum is chosen as simple equipment to calibrate particle–particle and particle–material interaction properties, as there is a lack of research on whether the rotary drum is adequate equipment to calibrate particle–material interaction properties. Therefore, this article calibrates particle–particle and particle–material static and rolling interaction coefficients using a rotary drum. The calibration of particle–material static and rolling friction coefficients are described using the rotating drum with a 45 degrees inclination. The particle–particle static and rolling friction coefficients were calibrated according to the angle of repose when the rotary drum is vertical.