The role of matrix metalloproteinase-2 and miR-196a2 in bronchial asthma pathogenesis and diagnosis
Osama A. Mohammed,
Ahmed S. Doghish,
Mohannad Mohammad S. Alamri,
Muffarah Hamid Alharthi,
Jaber Alfaifi,
Masoud I.E. Adam,
Abdullah Hassan Alhalafi,
AbdulElah Al Jarallah AlQahtani,
Assad Ali Rezigalla,
Magaji Garba Taura,
Adamu Imam Isa,
Ahad Fuad Binafif,
Mohammed A. Attia,
Elsayed A. Elmorsy,
Ayman A. Yousef,
Mustafa Ahmed Abdel-Reheim,
Mohamed A. Elkady
Affiliations
Osama A. Mohammed
Department of Pharmacology, College of Medicine, University of Bisha, Bisha, 61922, Saudi Arabia
Ahmed S. Doghish
Department of Biochemistry, Badr University in Cairo (BUC), Badr City, Cairo, 11829, Egypt; Department of Biochemistry and Molecular Biology, Al-Azhar University, Nasr City, Cairo, 11231, Egypt; Corresponding author. Department of Biochemistry, Badr University in Cairo (BUC), Badr City, Cairo, 11829, Egypt.
Mohannad Mohammad S. Alamri
Department of Family and Community Medicine, College of Medicine, University of Bisha, Bisha, 61922, Saudi Arabia
Muffarah Hamid Alharthi
Department of Family and Community Medicine, College of Medicine, University of Bisha, Bisha, 61922, Saudi Arabia
Jaber Alfaifi
Department of Child Health, College of Medicine, University of Bisha, Bisha, 61922, Saudi Arabia
Masoud I.E. Adam
Department of Medical Education and Internal Medicine, College of Medicine, University of Bisha, Bisha, 61922, Saudi Arabia
Abdullah Hassan Alhalafi
Department of Family and Community Medicine, College of Medicine, University of Bisha, Bisha, 61922, Saudi Arabia
AbdulElah Al Jarallah AlQahtani
Department of Internal Medicine, Division of Dermatology, College of Medicine, University of Bisha, Bisha, 61922, Saudi Arabia
Assad Ali Rezigalla
Department of Anatomy, College of Medicine, University of Bisha, Bisha, 61922, Saudi Arabia
Magaji Garba Taura
Department of Anatomy, College of Medicine, University of Bisha, Bisha, 61922, Saudi Arabia
Adamu Imam Isa
Department of Physiology, College of Medicine, University of Bisha, Bisha, 61922, Saudi Arabia
Ahad Fuad Binafif
Blood Transfusion Services Center, Health Support Services Center, Ministry of Health, Riyadh, 11176, Saudi Arabia
Mohammed A. Attia
Department of Clinical Pharmacology, Mansoura University, Mansoura, 35516, Egypt; Department of Basic Medical Sciences, College of Medicine, AlMaarefa University, Riyadh, 11597, Saudi Arabia
Elsayed A. Elmorsy
Department of Clinical Pharmacology, Mansoura University, Mansoura, 35516, Egypt; Department of Pharmacology and Therapeutics, College of Medicine, Qassim University, Buraydah, 51452, Saudi Arabia
Ayman A. Yousef
Chest Department, Benha University Hospitals, Qaliubyia, Egypt
Mustafa Ahmed Abdel-Reheim
Department of Pharmaceutical Sciences, College of Pharmacy, Shaqra University, Shaqra, 11961, Saudi Arabia; Department of Pharmacology and Toxicology, Beni-Suef University, Beni.Suef, 62521, Egypt; Corresponding author. Department of Pharmaceutical Sciences, College of Pharmacy, Shaqra University, Shaqra, 11961, Saudi Arabia.
Mohamed A. Elkady
Department of Biochemistry and Molecular Biology, Al-Azhar University, Nasr City, Cairo, 11231, Egypt
Background: Bronchial asthma is a persistent inflammatory respiratory condition that restricts the passage of air and causes hyperresponsiveness. Chronic asthma can be classified into three categories: mild, moderate, and severe. Remodeling took place as the extracellular matrix accumulated in the walls of the airways. Inflammation occurs as a result of the damage caused by matrix metalloproteinase-2 (MMP-2) to basement membrane type IV collagen. The severity of asthma may be associated with miR-196a2. The objective of our study was to investigate the underlying mechanisms and clinical relevance of miR-196a2 and MMP-2 serum levels in relation to the severity of asthma. Methods: This study recruited 85 controls and 95 asthmatics classified as mild, moderate, or severe. Expression of miR-196a2 was measured by quantitative reverse transcriptase PCR. Using the enzyme-linked immunosorbent assay (ELISA), MMP-2, IL-6, and total immunoglobulin E (IgE) levels in the serum of asthmatics of various grades were compared to a control group. MMP-2's diagnostic and prognostic potential was determined using ROC curve analysis. This study also measured blood Eosinophils and PFTs. We examined MMP-2's connections with IgE, blood Eosinophils, and PFTs. Results: The current investigation found that miR-196a2 expression was significantly higher in the control group than in asthmatic patients as a whole. The study found that severe asthmatics had higher MMP-2, IL-6, and IgE serum levels than healthy controls. We identified the MMP-2 serum concentration cutoff with great sensitivity and specificity. Significant relationships between MMP-2 serum level and miR-196a2 expression in the patient group with severe asthmatics were found. The MMP-2, IL-6, and IgE serum levels were considerably higher in mild, moderate, and severe asthmatics than controls. The miR-196a2 expression and MMP-2 serum concentration correlated positively with IgE and blood eosinophils % and negatively with all lung function tests in the asthmatic patient group.Conclusion: the study revealed that the elevated miR-196a2 expression and serum concentration of MMP-2, IL-6, and IgE associated with elevated blood eosinophils % is associated with pathophysiology and degree of asthma severity. The miR-196a2 expression and MMP-2 serum concentration have a promising diagnostic and prognostic ability in bronchial asthma.
