Identification of Chemical Markers for the Discrimination of Radix <i>Angelica sinensis</i> Grown in Geoherb and Non-Geoherb Regions Using UHPLC-QTOF-MS/MS Based Metabolomics
Kaixue Zhang,
Menglin Yan,
Shu Han,
Longfei Cong,
Liyao Wang,
Liu Zhang,
Lili Sun,
Haiying Bai,
Guanhua Wei,
Hong Du,
Min Jiang,
Gang Bai,
Zhigang Yang
Affiliations
Kaixue Zhang
School of Pharmacy, Lanzhou University, Lanzhou 730000, China
Menglin Yan
College of Pharmacy, Nankai University, State Key Laboratory of Medicinal Chemical Biology, Tianjin 300350, China
Shu Han
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China
Longfei Cong
College of Pharmacy, Nankai University, State Key Laboratory of Medicinal Chemical Biology, Tianjin 300350, China
Liyao Wang
School of Pharmacy, Lanzhou University, Lanzhou 730000, China
Liu Zhang
School of Pharmacy, Lanzhou University, Lanzhou 730000, China
Lili Sun
School of Pharmacy, Lanzhou University, Lanzhou 730000, China
Haiying Bai
School of Pharmacy, Lanzhou University, Lanzhou 730000, China
Guanhua Wei
School of Pharmacy, Lanzhou University, Lanzhou 730000, China
Hong Du
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, China
Min Jiang
College of Pharmacy, Nankai University, State Key Laboratory of Medicinal Chemical Biology, Tianjin 300350, China
Gang Bai
College of Pharmacy, Nankai University, State Key Laboratory of Medicinal Chemical Biology, Tianjin 300350, China
Zhigang Yang
School of Pharmacy, Lanzhou University, Lanzhou 730000, China
This research aimed to discover chemical markers for discriminating radix Angelica sinensis (RAS) from different regions and to explore the differences of RAS in the content of four active compounds and anti-inflammatory activities on lipopolysacchride (LPS)-induced RAW264.7 cells and calcium antagonists on the HEK 293T cells of RAS. Nine compounds were selected as characteristic chemical markers by ultra-high-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UHPLC-QTOF-MS/MS), based on metabolomics, in order to rapidly discriminate RAS from geoherb and non-geoherb regions. The contents of senkyunolide I and butylidenephthalide in geoherb samples were higher than those in non-geoherb samples, but the contents of ferulic acid and levistolide A were lower in the geoherb samples. Furthermore, the geoherbs showed better nitric oxide (NO) inhibitory and calcium antagonistic activities than the non-geoherbs. These results demonstrate the diversity in quality of RAS between geoherbs and non-geoherbs.