Revista Mexicana de Angiología (Jan 2022)

Expression of miR-145, miR-146, and miR-155 in an experimental model of arteriovenous fistula

  • Hugo Laparra-Escareño,
  • Alette Ortega-Gómez,
  • Joaquín Manzo-Merino,
  • Alejandro Zentella-Dehesa,
  • Luis O. Bobadilla-Rosado,
  • Carlos A. Hinojosa

DOI
https://doi.org/10.24875/RMA.22000001
Journal volume & issue
Vol. 50, no. 1

Abstract

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Background: The arteriovenous fistula (AVF) is the first option as vascular access for patients with end-stage renal disease. The main cause of failure is stenosis of the venous portion of the AVF secondary to intimal hyperplasia. Material and methods: The objective of this study was to evaluate the relative expression of miR-145, miR-146, and miR-155. We used an experimental model of intimal hyperplasia creating an AVF. We included six Wistar Rats. The analysis and validation of miR-145, miR-146, and miR-155 was performed using real-time polymerase chain reaction. Results: There is a down-regulation of miR-145 in the vein (0.233 ± 0.3, p = 0.0030) and artery (0.33 ± 0.4, p = 0.0630), compared to the control. Likewise, we found upregulation of miR-146 in the vein (29 ± 0.25, p = 0.0256) and artery (34.66 ± 0.35, p = 0.0345) compared to the control and upregulation of miR-155 in the vein (20 ± 0.39, p = 0.0231) and artery (22.66 ± 0.49, p = 0.0234) compared to the control. Conclusions: We found alterations in the expression of miR-145, miR-146, and miR-155 comparing the fistula versus control, both in the arterial and venous portions in an experimental model of AVF.

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