Blood Rheological Properties in Blood Loss (An experimental study)

Abstract

Blood loss is a complex pathological process with the mechanism that has not been fully unstudied. Blood rheology is a particular link that is involved in blood losses. Objective: to perform a comprehensive study of blood rheological proper ties, such as erythrocyte aggregability, erythrocyte deformability, plasma viscosity, and packed cell volume, in different degrees of experimental blood loss. Materials and methods. Blood loss of different stages was simulated in anesthetized animals. The Georgian technique, membrane filtration, a capillary method, and a standard centrifugation method were used to study blood rheological properties. The data were analyzed applying the statistical programs Origin 4.1 (Microcat.Software.Inc) and Microsoft Excel. Results. In stages 1, 2, and 3 blood losses, erythrocyte aggregability was significantly increased by 10, 25, and 44% and erythrocyte deformability was decreased by 12, 13, and 15%, respectively, as compared to the controls. The delta viscosity was insignificant (both in the subgroups in different blood loss degreesversus the control group); the packed cell volume in blood loss was increased insignificantly as compared to the control.Conclusion. Comprehensive assessment of the degree of blood loss requires a minimum set of blood rheological indices, such as erythrocyte aggregability and deformability, blood viscosity, and packed cell volume. The basis for monitoring the rheological indices of massive blood loss is the specific features of erythrocyte aggregation.

Keywords

• blood rheology
• erythrocyte aggregability
• blood loss