Acta Cirúrgica Brasileira (Dec 2022)

Tissue healing changes on wounds in rats after treatment with Hancornia speciosa latex in cream-gel formulation

  • Patrícia Lima D’Abadia,
  • Susy Ricardo Lemes,
  • Paulo Roberto de Melo-Reis,
  • Ruy de Souza Lino Júnior,
  • Pablo José Gonçalves,
  • Diego dos Santos Reis,
  • Graziele Alícia Batista Caixeta,
  • Vanessa Cristine Santana Amaral,
  • Luciane Madureira Almeida

DOI
https://doi.org/10.1590/acb371001
Journal volume & issue
Vol. 37, no. 10

Abstract

Read online

ABSTRACT Purpose: Hancornia speciosa latex has shown pharmacological potential in wound healing processes due to its angiogenic, osteogenic, and anti-inflammatory activities. The aims of this study were to carry out a cream-gel formulation with 5, 10 and 25% of H. speciosa serum latex and to evaluate its potential to stimulate the skin regeneration in rats’ wounds. Methods: One hundred and twenty rats were divided into five groups: neutral control with saline (G1), cream-gel based on H. speciosa latex serum at 5% m/v (G2), cream-gel at 15% m/v (G3), cream-gel at 25% m/v (G4), and cream-gel (G5). The animals were euthanized at three, seven, 14 and 21 days after the injury induction, and some parameters were analyzed: wound contraction, necrosis, fibrin, polymorphonuclear and mononuclear infiltrates, fibroblast, angiogenesis, hemorrhage, and collagen. Results: The therapeutic treatment with cream-gel at 15 and 25% is beneficial in the inflammatory phase of healing processes since it increased the angiogenesis and proliferation of mononuclear infiltrations in wounds. Regarding wound contraction, the treatment with cream-gel (5 and 15%) induced a higher rate of contraction in the proliferative phase. The 15% cream-gel formulation stimulated a greater production of collagen in the injured tissues. Conclusions: H. speciosa cream-gel is a low-cost herbal medicine which can aid in tissue repair.

Keywords