International Journal of Molecular Sciences (Jan 2022)

Benzyl Isothiocyanate Attenuates Inflammasome Activation in <i>Pseudomonas aeruginosa</i> LPS-Stimulated THP-1 Cells and Exerts Regulation through the MAPKs/NF-κB Pathway

  • Won Sun Park,
  • Jeonghan Lee,
  • Giyoun Na,
  • SaeGwang Park,
  • Su-Kil Seo,
  • Jung Sik Choi,
  • Won-Kyo Jung,
  • Il-Whan Choi

DOI
https://doi.org/10.3390/ijms23031228
Journal volume & issue
Vol. 23, no. 3
p. 1228

Abstract

Read online

Inflammasomes are a group of intracellular multiprotein platforms that play important roles in immune systems. Benzyl isothiocyanate (BITC) is a constituent of cruciferous plants and has been confirmed to exhibit various biological activities. The modulatory effects of BITC on inflammasome-mediated interleukin (IL)-1β expression and its regulatory mechanisms in Pseudomonas aeruginosa (P. aeruginosa) LPS/ATP-stimulated THP-1 cells was investigated. Monocytic THP-1 cells were treated with phorbol myristate acetate (PMA) to induce differentiation into macrophages. Enzyme-linked immunosorbent assays (ELISA) were performed to measure the levels of IL-1β produced in P. aeruginosa LPS/ATP-exposed THP-1 cells. Western blotting was performed to examine the BITC modulatory mechanisms in inflammasome-mediated signaling pathways. BITC inhibited IL-1β production in P. aeruginosa LPS/ATP-induced THP-1 cells. BITC also inhibited activation of leucine-rich repeat protein-3 (NLRP3) and caspase-1 in P. aeruginosa LPS/ATP-induced THP-1 cells. Furthermore, we show that mitogen-activated protein kinase (MAPK) and nuclear factor-κB (NF-κB) activation in P. aeruginosa LPS was attenuated by BITC. These BITC-mediated modulatory effects on IL-1β production may have therapeutic potential for inflammasome-mediated disorders such as a nasal polyp.

Keywords