Fabrication and application of double-layer nanopore

Abstract

Solid-state nanopores have become the most promising single-molecule sequencing tools due to their adjustable pore size, stable physical and chemical properties, strong adaptability to extreme environments, and high integration. In the area of solid-state nanopores research, improving the detection accuracy of single molecules has always been the core concern of researchers. In recent years, the double-layer nanopores has attracted wide attention. Compared with traditional single-layer nanopore, the "pore-cavity-pore" structure of double-layer nanopores provides two molecular recognition sites and nanoconfined space. The two molecular recognition sites provided by the two nanopores can obtain two target signals in a single translocation event, which not only enrich the detection information but also provide the most direct comparison information source for signal analysis. In addition, the "cavity" in the double-layer nanopores can be used as a single-molecule chemical reactor. Therefore, the appearance of double-layer nanopores broadens the application range of nanopore sensors and has an important application prospect in single molecule detection. In this paper, the development history of nanopores is summarized, and the fabrication methods of double-layer nanopores and their applications in the field of single-molecule detection are emphatically introduced.

Keywords

• solid-state nanopores
• double-layer nanopore
• double detection signal
• fabrication methods