Gene Expression Profiles of Rodent Atrophic Gastritis Induced By Hot and Salt Water

Abstract

Diet factors may be potential causes for atrophic gastritis. This study is to establish rat atrophic gastritis models under various hot and salt water conditions and to explore the associated molecular mechanisms. 96 SD rats were divided randomly into 4 experimental groups and used to establish atrophic gastritis models. 2 rats from each group were sacrificed every other week to collect gastric sinus tissues for pathological analysis. When atrophic lesion was identified in a given group, all remaining rats in that group were sacrificed and gastric sinus tissues were collected. The cDNA probes from sinus atrophic lesion or control sinus mucous were labeled with Cy5 or Cy3, respectively. These probes were mixed and hybridized with cDNA microarrays. Hot salt water group was pathologically confirmed to exhibit atrophic lesion in 10 weeks. Salt water group and hot water group were confirmed to exhibit atrophic lesion in 24 weeks. The atrophic lesions located mainly in gastric sinus. 288 differentially expressed genes were identified between hot salt water group and normal control group. 162 differentially expressed genes were identified between hot water group and normal control group. 81 differentially expressed genes were identified between hot salt water group and salt water group. In conclusion, rat atrophic gastritis models induced by various hot and salt water conditions have been established. The corresponding gene expression profiles have been firstly established. This study shows that dietary factors such as temperature and salt concentration may play an important role in the development of atrophic gastritis.

Keywords

• rat
• atrophic gastritis model
• gene expression profiling
• cdna microarrays
• hybridization