MOLECULAR VARIABILITY OF THE COAT PROTEIN GENE OF Prunus necrotic ringspot virus ISOLATES

Abstract

We have obtained and described the nucleotide sequences corresponding to ilarvirus Prunus necrotic ringspot virus (PNRSV] coat protein (CP) gene in eleven Polish isolates, from different Prunus and Rosa species and four isolates provided in plant material from Australia, Hungary and Italy. Virus identification was possible using specific primers allowed amplifying the 700 bp amplicon of coat protein gene. The product was obtained used IC-RT-PCR. The results indicated no association between the host species or the geographic origin and the PNRSV CP sequence specificity. The CP gene nucleotide sequence of studied isolates allowed for clustering them into the previously reported PV32-I, PV96-II and PE5-III phylogroups.

Keywords

• PNRSV
• CP
• IC-RT-PCR
• sequencing