Identification of Pathogen Causing Bulb Rot in <i>Fritillaria taipaiensis</i> P. Y. Li and Establishment of Detection Methods

Shijie Wang; Keke Chen; Jiaqi Guo; Panwang Zhang; Yuchen Li; Zhenghao Xu; Langjun Cui; Yi Qiang

doi:10.3390/plants13162236

Plants (Aug 2024)

Identification of Pathogen Causing Bulb Rot in <i>Fritillaria taipaiensis</i> P. Y. Li and Establishment of Detection Methods

Shijie Wang,
Keke Chen,
Jiaqi Guo,
Panwang Zhang,
Yuchen Li,
Zhenghao Xu,
Langjun Cui,
Yi Qiang

Affiliations

Shijie Wang: National Engineering Laboratory for Resource Development of Endangered Chinese Crude Drugs in Northwest of China, Shaanxi Normal University, Xi’an 710119, China
Keke Chen: School of Biological and Environmental Engineering, Xi’an University, Xi’an 710065, China
Jiaqi Guo: National Engineering Laboratory for Resource Development of Endangered Chinese Crude Drugs in Northwest of China, Shaanxi Normal University, Xi’an 710119, China
Panwang Zhang: National Engineering Laboratory for Resource Development of Endangered Chinese Crude Drugs in Northwest of China, Shaanxi Normal University, Xi’an 710119, China
Yuchen Li: National Engineering Laboratory for Resource Development of Endangered Chinese Crude Drugs in Northwest of China, Shaanxi Normal University, Xi’an 710119, China
Zhenghao Xu: National Engineering Laboratory for Resource Development of Endangered Chinese Crude Drugs in Northwest of China, Shaanxi Normal University, Xi’an 710119, China
Langjun Cui: National Engineering Laboratory for Resource Development of Endangered Chinese Crude Drugs in Northwest of China, Shaanxi Normal University, Xi’an 710119, China
Yi Qiang: National Engineering Laboratory for Resource Development of Endangered Chinese Crude Drugs in Northwest of China, Shaanxi Normal University, Xi’an 710119, China

DOI: https://doi.org/10.3390/plants13162236
Journal volume & issue: Vol. 13, no. 16
p. 2236

Abstract

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Fritillaria taipaiensis P. Y. Li (F. taipaiensis) is a traditional Chinese herbal medicine that has been used for over two millennia to treat cough and expectoration. However, the increasing cultivation of F. taipaiensis has led to the spread of bulb rot diseases. In this study, pathogens were isolated from rotten F. taipaiensis bulbs. Through molecular identification, pathogenicity testing, morphological assessment, and microscopy, Fusarium solani was identified as the pathogen causing bulb rot in F. taipaiensis. The colonization of F. solani in the bulbs was investigated through microscopic observation. The rapid and accurate detection of this pathogen will contribute to better disease monitoring and control. Loop-mediated isothermal amplification (LAMP) and qPCR methods were established to quickly and specifically identify this pathogen. These results provide valuable insights for further research on the prediction, rapid detection, and effective prevention and control of bulb rot in F. taipaiensis.

Published in Plants

ISSN: 2223-7747 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Botany
Website: http://www.mdpi.com/journal/plants

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