PLoS ONE (Jan 2016)

Identification of Novel Sequence Types among Staphylococcus haemolyticus Isolated from Variety of Infections in India.

  • Sasmita Panda,
  • Smrutiti Jena,
  • Savitri Sharma,
  • Benu Dhawan,
  • Gopal Nath,
  • Durg Vijai Singh

DOI
https://doi.org/10.1371/journal.pone.0166193
Journal volume & issue
Vol. 11, no. 11
p. e0166193

Abstract

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The aim of this study was to determine sequence types of 34 S. haemolyticus strains isolated from a variety of infections between 2013 and 2016 in India by MLST. The MEGA5.2 software was used to align and compare the nucleotide sequences. The advanced cluster analysis was performed to define the clonal complexes. MLST analysis showed 24 new sequence types (ST) among S. haemolyticus isolates, irrespective of sources and place of isolation. The finding of this study allowed to set up an MLST database on the PubMLST.org website using BIGSdb software and made available at http://pubmlst.org/shaemolyticus/. The data of this study thus suggest that MLST can be used to study population structure and diversity among S. haemolyticus isolates.