Generation of an induced pluripotent stem cell line (SHETi004-A) from a Chinese Han child with left bundle branch block

Tingxia Wang; Shan Hou; Juan Chen; Xiaopei Zhao; Tingting Xiao; Cuilan Hou; Cuizhen Zhou

Stem Cell Research (Dec 2023)

Generation of an induced pluripotent stem cell line (SHETi004-A) from a Chinese Han child with left bundle branch block

Tingxia Wang,
Shan Hou,
Juan Chen,
Xiaopei Zhao,
Tingting Xiao,
Cuilan Hou,
Cuizhen Zhou

Affiliations

Tingxia Wang: Department of Cardiology, Shanghai Children’s Hospital, School of medicine, Shanghai Jiaotong University, No. 355 Luding Road, Shanghai, 200062, China
Shan Hou: Department of Cardiology, Shanghai Children’s Hospital, School of medicine, Shanghai Jiaotong University, No. 355 Luding Road, Shanghai, 200062, China
Juan Chen: Department of Cardiology, Shanghai Children’s Hospital, School of medicine, Shanghai Jiaotong University, No. 355 Luding Road, Shanghai, 200062, China
Xiaopei Zhao: Department of Cardiology, Shanghai Children’s Hospital, School of medicine, Shanghai Jiaotong University, No. 355 Luding Road, Shanghai, 200062, China
Tingting Xiao: Department of Cardiology, Shanghai Children’s Hospital, School of medicine, Shanghai Jiaotong University, No. 355 Luding Road, Shanghai, 200062, China
Cuilan Hou: Department of Cardiology, Shanghai Children’s Hospital, School of medicine, Shanghai Jiaotong University, No. 355 Luding Road, Shanghai, 200062, China; NHC Key Laboratory of Medical Embryogenesis and Developmental Molecular Biology, Shanghai Key Laboratory of Embryo and Reproduction Engineering, Shanghai, 200062, China; Corresponding authors at: Department of Cardiology, Shanghai Children’s Hospital, School of medicine, Shanghai Jiaotong University, No. 355 Luding Road, Shanghai 200062, China.
Cuizhen Zhou: Department of Cardiology, Shanghai Children’s Hospital, School of medicine, Shanghai Jiaotong University, No. 355 Luding Road, Shanghai, 200062, China; Corresponding authors at: Department of Cardiology, Shanghai Children’s Hospital, School of medicine, Shanghai Jiaotong University, No. 355 Luding Road, Shanghai 200062, China.

Journal volume & issue: Vol. 73
p. 103227

Abstract

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Desmin (DES) is an important intermediate filament protein associated with the extrasarcomeric cytoskeleton and cellular function that was first reported to be associated with cardiac conduction disease and cardiomyopathy in 1998. We generated an induced pluripotent stem cell (iPSC) line from the left bundle branch block (LBBB) patient’s peripheral blood mononuclear cells using Sendai virus-mediated reprogramming. The iPSCs exhibited stable amplification, expressed pluripotent markers, and spontaneously differentiated into three layers in vitro. Additionally, it showed a normal diploid karyotype and maintained the pathogenic mutation in DES. Hence, the iPSC line provided a platform for exploring LBBB mechanisms associated with DES mutations.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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